Genome-wide investigation of the OR gene family in Helicoverpa armigera and functional analysis of OR48 and OR75 in metamorphosis development.

Autor: Li Y; Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China., Chai Q; Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China., Chen Y; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China., Ma Y; College of Life Sciences, Shandong Normal University, Jinan 250300, Shandong, China., Wang Y; Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China., Zhao J; Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China. Electronic address: zhaojunsheng@saas.ac.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Oct; Vol. 278 (Pt 1), pp. 134646. Date of Electronic Publication: 2024 Aug 10.
DOI: 10.1016/j.ijbiomac.2024.134646
Abstrakt: The cotton bollworm, Helicoverpa armigera, is a significant global agricultural pest, particularly detrimental during its larval feeding period. Insects' odorant receptors (ORs) are crucial for their crop-feeding activities, yet a comprehensive analysis of H. armigera ORs has been lacking, and the influence of hormones on ORs remain understudied. Herein, we conducted a genome-wide study and identified 81 ORs, categorized into 15 distinct groups. Analyses of protein motifs and gene structures revealed both conservation within groups and divergence among them. Comparative gene duplication analysis between H. armigera and Bombyx mori highlighted different duplication patterns. We further investigated subcellular localization and protein interactions within the odorant receptor family, providing valuable insights for future functional and interaction studies of ORs. Specifically, we identified that OR48 and OR75 were abundantly expressed during molting/metamorphosis and feeding stages, respectively. We demonstrated that 20E induced the upregulation of OR48 via EcR, while insulin upregulated OR75 expression through InR. Moreover, 20E induced the translocation of OR48 to the cell membrane, mediating its effects. Functional studies involving the knockdown of OR48 and OR75 revealed their roles in metamorphosis development, with OR48 knockdown resulting in delayed pupation and OR75 knockdown leading to premature pupation. OR48 can promote autophagy and apoptosis in fat body, while OR75 can significantly inhibit apoptosis and autophagy. These findings significantly contribute to our understanding of OR function in H. armigera and shed light on potential avenues for pest control strategies.
Competing Interests: Declaration of competing interest The authors have no conflict of interest to declare.
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Databáze: MEDLINE
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