Antimicrobial effects of epigallocatechin-3-gallate, a catechin abundant in green tea, on periodontal disease-associated bacteria.

Autor: Higuchi M; Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Japan., Abiko Y; Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Japan., Washio J; Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Japan., Takahashi N; Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Japan. Electronic address: nobuhiro.takahashi.a5@tohoku.ac.jp.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2024 Nov; Vol. 167, pp. 106063. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1016/j.archoralbio.2024.106063
Abstrakt: Objective: Epigallocatechin-3-gallate (EGCG), a catechin abundant in green tea, exhibits antibacterial activity. In this study, the antimicrobial effects of EGCG on periodontal disease-associated bacteria (Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Fusobacterium nucleatum, and Fusobacterium periodontium) were evaluated and compared with its effects on Streptococcus mutans, a caries-associated bacterium.
Results: Treatment with 2 mg/ml EGCG for 4 h killed all periodontal disease-associated bacteria, whereas it only reduced the viable count of S. mutans by about 40 %. Regarding growth, the periodontal disease-associated bacteria were more susceptible to EGCG than S. mutans, based on the growth inhibition ring test. As for metabolism, the 50 % inhibitory concentration (IC 50 ) of EGCG for bacterial metabolic activity was lower for periodontal disease-associated bacteria (0.32-0.65 mg/ml) than for S. mutans (1.14 mg/ml). Furthermore, these IC 50 values were negatively correlated with the growth inhibition ring (r = -0.73 to -0.86). EGCG induced bacterial aggregation at the following concentrations: P. gingivalis (>0.125 mg/ml), F. periodonticum (>0.5 mg/ml), F. nucleatum (>1 mg/ml), and P. nigrescens (>2 mg/ml). S. mutans aggregated at an EGCG concentration of > 1 mg/ml.
Conclusion: EGCG may help to prevent periodontal disease by killing bacteria, inhibiting bacterial growth by suppressing bacterial metabolic activity, and removing bacteria through aggregation.
Competing Interests: Declaration of Competing Interest The authors declare that there are no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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