Protocol for fluorescent live-cell staining of tardigrades.

Autor:	Harry CJ; Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Hibshman JD; Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA., Damatac A 2nd; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Max Planck Institute for Evolutionary Biology, 24306 Ploen, Germany., Davidson PL; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Biology, Indiana University, Bloomington, IN 47405, USA., Estermann MA; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Reproductive Developmental Biology Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA., Flores-Flores M; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; University of Colorado, School of Medicine, Anschutz Medical Campus, Department of Pediatrics, Section of Developmental Biology, 12801 E 17th Avenue, Aurora, CO 80045, USA., Holmes CM; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Organismal and Evolutionary Biology, Harvard University, Boston, MA, USA., Lázaro J; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; European Molecular Biology Laboratory (EMBL) Barcelona, Dr. Aiguader 88, 08003 Barcelona, Spain., Legere EA; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Human Genetics, McGill University, Montreal, QC, Canada., Leyhr J; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Organismal Biology, Uppsala University, Uppsala, Sweden; Department of Biology, Duke University, Durham, NC 27708, USA., Thendral SB; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Biology, Duke University, Durham, NC 27708, USA., Vincent BA; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Ecology, Evolution, and Marine Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA., Goldstein B; Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA. Electronic address: bobg@unc.edu.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103232. Date of Electronic Publication: 2024 Aug 09.
DOI:	10.1016/j.xpro.2024.103232
Abstrakt:	Tardigrades are microscopic organisms with exceptional resilience to environmental extremes. Most protocols to visualize the internal anatomy of tardigrades rely on fixation, hampering our understanding of dynamic changes to organelles and other subcellular components. Here, we provide protocols for staining live tardigrade adults and other postembryonic stages, facilitating real-time visualization of structures including lipid droplets, mitochondria, lysosomes, and DNA. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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