Hemoplasmas in wild rodents and marsupials from the Caatinga Biome, Brazil.

Autor: Torres-Santos PT; Parasitic Diseases Laboratory, Federal University of the São Francisco Valley, Petrolina, Pernambuco, Brasil; Graduate Program in Veterinary Sciences in the Semiarid Region, Federal University of Vale do São Francisco, Petrolina, Pernambuco, Brazil., Evaristo AMDCF; Parasitic Diseases Laboratory, Federal University of the São Francisco Valley, Petrolina, Pernambuco, Brasil; Graduate Program in Veterinary Sciences in the Semiarid Region, Federal University of Vale do São Francisco, Petrolina, Pernambuco, Brazil., Santos JR; 72° Batalhão de Infantaria Motorizado, 72° BIMtz., Petrolina, Pernambuco, Brasil., Collere FCM; Graduate Program in Veterinary Sciences, Universidade Federal do Paraná - UFPR, Curitiba, Paraná, Brasil., Vieira TSWJ; Center for Computational Intelligence to Predict Health and Environmental Risks (CIPHER), The University of North Carolina at Charlotte, Charlotte, USA., Pereira LCM; Center Fauna Management, Cema-Fauna, Federal University of the São Francisco Valley, Petrolina, Pernambuco, Brazil., Nicola PA; Center Fauna Management, Cema-Fauna, Federal University of the São Francisco Valley, Petrolina, Pernambuco, Brazil., Vieira RFDC; Center for Computational Intelligence to Predict Health and Environmental Risks (CIPHER), The University of North Carolina at Charlotte, Charlotte, USA; Department of Epidemiology and Community Health, The University of North Carolina at Charlotte, Charlotte, USA., Horta MC; Parasitic Diseases Laboratory, Federal University of the São Francisco Valley, Petrolina, Pernambuco, Brasil; Graduate Program in Veterinary Sciences in the Semiarid Region, Federal University of Vale do São Francisco, Petrolina, Pernambuco, Brazil. Electronic address: mauricio.horta@univasf.edu.br.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2024 Oct; Vol. 258, pp. 107334. Date of Electronic Publication: 2024 Aug 08.
DOI: 10.1016/j.actatropica.2024.107334
Abstrakt: A total of 231 blood samples from wild mammals belonging to the orders Rodentia (n = 142) and Didelphimorphia (n = 89) were screened by real-time PCR assay (qPCR), being six Rhipidomys sp., 118 Thrichomys laurentius, nine Rattus rattus, four Kerodon rupestris, five Necromys lasiurus, 42 Didelphis albiventris and 47 Monodelphis domestica. Results using qPCR showed that 32 of the total 231 (13.85 %) samples were positive for hemoplasma sequences of the 16S rRNA gene. Sequences from two D. albiventris showed 99.77-99.89 % identity with 'Candidatus Mycoplasma haemoalbiventris' and 99.09 % with 'Candidatus Mycoplasma haemodidelphidis', respectively. Furthermore, one M. domestica and five T. laurentius showed 99.72-99.77 % identity with Mycoplasma sp., and one K. rupestris showed 98.13 % identity with 'Candidatus Mycoplasma haematohydrochaerus'; and from two Rattus rattus showed 99.65-99.89 % identity with Mycoplasma sp. and 'Candidatus Mycoplasma haemomuris'. The 23S rRNA gene sequences obtained from the two D. albiventris showed 100 % identity with 'Ca. M. haemoalbiventris' whereas the sequences from the R. rattus showed only 85.31 % identity with 'Candidatus Mycoplasma haematohydrochaerus'. Two T. laurentius and one K. rupestris showed 84.66-92.97 % identity with 'Candidatus Mycoplasma haemosphiggurus'. Based on phylogenetic and Neighbor-Net network analyses of the 16S and 23S rRNA genes, potential novel species are described. In addition, 'Ca. M. haemoalbiventris' was detected in Didelphis albiventris, and Mycoplasma sp. was detected in Rattus sp. rodents from the Caatinga biome, Brazil.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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