Polymicrobial consortia in the pathogenesis of biofilm vaginosis visualized by FISH. Historic review outlining the basic principles of the polymicrobial infection theory.

Autor: Swidsinski A; Moleculargenetic Laboratory for Polymicrobial Infections and Biofilms, Universitätsmedizin Berlin CCM Charité, 10098 Germany; I.M. Sechenov First Moscow State Medical University, Institute of Public Health, Department of General Hygiene, 119991 Moscow, Russia. Electronic address: alexander.swidsinski@charite.de., Amann R; Max Planck Institute for Marine Microbiology, Department of Molecular Ecology, Celsiusstr. 1 D-28359 Bremen, Germany. Electronic address: ramann@mpi-bremen.de., Guschin A; Molecular-genetic Laboratory, Division Polymicrobial Infections. Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology, 119071 Moscow, Russia. Electronic address: alegus65@mail.ru., Swidsinski S; Moleculargenetic Laboratory for Polymicrobial Infections and Biofilms, Universitätsmedizin Berlin CCM Charité, 10098, Germany. Electronic address: sswidsinski@yahoo.de., Loening-Baucke V; Moleculargenetic Laboratory for Polymicrobial Infections and Biofilms, Universitätsmedizin Berlin CCM Charité, 10098, Germany. Electronic address: veraloening@gmail.com., Mendling W; Das Deutsche Zentrum für Infektionen in Gynäkologie und Geburtshilfe, Landesfrauenklinik Helios Universitätsklinikum Wuppertal - Universität Witten/Herdecke, Heusnerstraße 40, 42283 Wuppertal, Germany. Electronic address: w.mendling@t-online.de., Sobel JD; Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Mk. 48322, USA. Electronic address: jsobel@med.wayne.edu., Lamont RF; BSc MB ChB DM FRCOG University of Southern Denmark, Odense, Denmark; Northwick Park Institute for Medical Research Campus, University College London, UK. Electronic address: rflamont@icloud.com., Vaneechoutte M; Laboratory Bacteriology Research Department Diagnostic Sciences GE32, Faculty of Medicine & Health Sciences Ghent University, Flanders, Belgium; Medical Research Building II, 1st Floor Ghent University Hospital, Entrance 38 C. Heymanslaan 10, 9000 GENT, Belgium. Electronic address: Mario.vaneechoutte@ugent.be., Baptista PV; Hospital Lusíadas Porto, Porto, Portugal; Department of Gynecology-Obstetrics and Pediatrics, Faculdade de Medicina da Universidade Do Porto, Porto, Portugal. Electronic address: pedrovieirabaptista@gmail.com., Bradshaw CS; School of Translational Medicine, Monash University, Melbourne, Victoria, UK; Australia Melbourne Sexual Health Centre, Alfred Hospital, Carlton, Victoria, Australia; Melbourne School of Population and 99 Global Health, University of Melbourne, Parkville, Victoria, Australia. Electronic address: catriona.bradshaw@monash.edu., Kogan IY; D.O. Ott Res.Inst Ob/Gyn/ Rep, 199034, St. Petersburg, Russia. Electronic address: ikogan@mail.ru., Savicheva АM; Department of Medical Microbiology, D.O. Ott Res.Inst Ob/Gyn/ Rep, 199034, St. Petersburg, Russia; St. Petersburg State Pediatric Med. University, 194100, St. Petersburg, Russia; Center for Resistance of Polymicrobial Communities and Biofilms, Russia. Electronic address: savitcheva@mail.ru., Mitrokhin OV; I.M. Sechenov First Moscow State Medical University, Institute of Public Health, Department of General Hygiene, 119991 Moscow, Russia. Electronic address: mov1163@yandex.ru., Swidsinski NW; Laboratory for Ecology and Clinical Implications of Polymicrobial Infections I.M. Sechenov First Moscow State Medical University, Institute of Public Health, Department of General Hygiene, 119991 Moscow, Russia. Electronic address: svidzinskaya_n_v@staff.sechenov.ru., Sukhikh GT; Microbiological Laboratory for Polymicrobial Infections and Biofilms. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia. Electronic address: sekretariat@oparina4.ru., Priputnevich TV; Microbiological Laboratory for Polymicrobial Infections and Biofilms. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia. Electronic address: Priput1@gmail.com., Apolikhina IA; Clinic Division for Polymicrobial Infections in Gynaecology, Department of Aesthetic Gynecology and Rehabilitation, Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Russia. Electronic address: apolikhina@inbox.ru., Dörffel Y; Outpatient Clinic, Charité Universitätsmedizin Berlin, CCM, 10117 Berlin, Germany. Electronic address: yvonne.doerffel@charite.de.
Jazyk: angličtina
Zdroj: Microbes and infection [Microbes Infect] 2024 Aug 08, pp. 105403. Date of Electronic Publication: 2024 Aug 08.
DOI: 10.1016/j.micinf.2024.105403
Abstrakt: The manuscript disputes the exclusive mono-infectious way of thinking, which presumes that for every infection only one pathogen is responsible and sufficient, when infectious vectors, close contact and reduced immunity meet. In situations involving heavily colonized anatomical sites such an approach often ends in insoluble contradictions. Upon critical reflection and evaluation of 20 years research on spatial organization of vaginal microbiota it is apparent, that in some situations, pathogens may act and operate in permanent, structurally organized consortia, whereas its individual components may be innocuous and innocent, failing to express any pathogenic effect. In these cases, consortia are the true pathogens responsible for many infectious conditions, which usually remain unrecognized as long as improperly diagnosed. The structure of such consortia can be unraveled using ribosomal fluorescence in situ hybridization (FISH). FISH methodology, that not only offers an ex vivo opportunity to recognize bacterial species, but provides unique physical insight into their specific role in the pathogenesis of polymicrobial infections. Ribosomal FISH technique applied to both, women with bacterial vaginosis (BV) and their male partners, has added significantly to our understanding of the pathogenesis of this condition and contributed to appreciating the mechanisms of polymicrobial, community-based infection, potentially leading to therapeutic advances.
Competing Interests: Declaration of competing interest The authors have no conflict of interest (including financial commitments or ethical concerns). The data of this publication were not presented previously. The work has not been published previously and is not under consideration for publication elsewhere. No generative artificial intelligence was used. The publication is approved by all authors and tacitly or explicitly by the responsible authorities where the work was carried out. If accepted, it will not be published elsewhere in the same form, in English or in any other language, including electronically without the written consent of the copyright-holder.
(Copyright © 2024. Published by Elsevier Masson SAS.)
Databáze: MEDLINE
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