Matrix design for optimal pancreatic β cells transplantation.

Autor: Rajkumari N; Laboratory of Fundamental and Applied Bioenergetics (LBFA), Grenoble Alpes University and INSERM U1055, France; Nantes University, CRCI2NA, INSERM 1307, 44000 Nantes, France. Electronic address: nikita.rajkumari@etu.univ-nantes.fr., Shalayel I; SyNaBi & Platform of Intravital Microscopy, TIMC-IMAG, CNRS UMR 5525, Grenoble Alpes University, Grenoble INP, INSERM, Grenoble, France. Electronic address: ibrahim.shalayel@univ-grenoble-alpes.fr., Tubbs E; Grenoble Alpes University, CEA, INSERM, IRIG, 38000 Grenoble, Biomics, France. Electronic address: TUBBS@cea.fr., Perrier Q; Univ. Grenoble Alpes, INSERM, Grenoble Alpes University Hospital, Department of Pharmacy, LBFA U1055, Grenoble, France. Electronic address: qperrier@chu-grenoble.fr., Chabert C; Laboratory of Fundamental and Applied Bioenergetics (LBFA), Grenoble Alpes University and INSERM U1055, France. Electronic address: clovis.chabert@univ-grenoble-alpes.fr., Lablanche S; Laboratory of Fundamental and Applied Bioenergetics (LBFA), Grenoble Alpes University and INSERM U1055, France; Department of Endocrinology-Diabetology-Nutrition, Grenoble University Hospital, France. Electronic address: SLablanche@chu-grenoble.fr., Benhamou PY; Laboratory of Fundamental and Applied Bioenergetics (LBFA), Grenoble Alpes University and INSERM U1055, France; Department of Endocrinology-Diabetology-Nutrition, Grenoble University Hospital, France. Electronic address: PYBenhamou@chu-grenoble.fr., Arnol C; SyNaBi & Platform of Intravital Microscopy, TIMC-IMAG, CNRS UMR 5525, Grenoble Alpes University, Grenoble INP, INSERM, Grenoble, France., Gredy L; MoVe, Laboratoire interdisciplinaire de physique, CNRS UMR 5588, Grenoble Alpes University, St-Martin d'Hères, France. Electronic address: laetitia.gredy@univ-grenoble-alpes.fr., Divoux T; ENSL, CNRS, Laboratoire de Physique, F-69342 Lyon, France. Electronic address: thibaut.divoux@ens-lyon.fr., Stephan O; MoVe, Laboratoire interdisciplinaire de physique, CNRS UMR 5588, Grenoble Alpes University, St-Martin d'Hères, France. Electronic address: olivier.stephan@univ-grenoble-alpes.fr., Zebda A; SyNaBi & Platform of Intravital Microscopy, TIMC-IMAG, CNRS UMR 5525, Grenoble Alpes University, Grenoble INP, INSERM, Grenoble, France. Electronic address: abdelkader.zebda@univ-grenoble-alpes.fr., van der Sanden B; SyNaBi & Platform of Intravital Microscopy, TIMC-IMAG, CNRS UMR 5525, Grenoble Alpes University, Grenoble INP, INSERM, Grenoble, France. Electronic address: boudewijn.vandersanden@univ-grenoble-alpes.fr.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2024 Nov; Vol. 164, pp. 213980. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1016/j.bioadv.2024.213980
Abstrakt: New therapeutic approaches to treat type 1 diabetes mellitus relies on pancreatic islet transplantation. Here, developing immuno-isolation strategies is essential to eliminate the need for systemic immunosuppression after pancreatic islet grafts. A solution is the macro-encapsulation of grafts in semipermeable matrixes with a double function: separating islets from host immune cells and facilitating the diffusion of insulin, glucose, and other metabolites. This study aims to synthesize and characterize different types of gelatin-collagen matrixes to prepare a macro-encapsulation device for pancreatic islets that fulfill these functions. While natural polymers exhibit superior biocompatibility compared to synthetic ones, their mechanical properties are challenging to reproduce. To address this issue, we conducted a comparative analysis between photo-crosslinked gelatin matrixes and chemically crosslinked collagen matrixes. We show that the different crosslinkers and polymerization methods influence the survival and glucose-stimulated insulin production of pancreatic β cells (INS1) in vitro, as well as the in vitro and in vivo stability of the matrix and the immuno-isolation in vivo. Among the matrixes, the stiff multilayer GelMA matrixes (8.5 kPa), fabricated by digital light processing, were the best suited for pancreatic β cells macro-encapsulation regarding these parameters. Within the alveoli of this matrix, pancreatic β cells spontaneously formed aggregates.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. B. van der Sanden, A. Zebda, N. Rajkumari, I. Shalayel, S. Lablanche reports financial support was provided by National Centre for Scientific Research. B. van der Sanden, A. Zebda report a relationship with INSERM that includes: employment. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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