Temporal Analysis of DSB Repair Outcome in Caenorhabditis elegans Meiosis.
| Autor: | Hicks T; Department of Biology, The University of Iowa, Iowa City, IA, USA., Silva N; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. silva@med.muni.cz., Smolikove S; Department of Biology, The University of Iowa, Iowa City, IA, USA. sarit-smolikove@uiowa.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2818, pp. 195-212. |
| DOI: | 10.1007/978-1-0716-3906-1_13 |
| Abstrakt: | The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID) system allows can unveil new/unappreciated roles for critical meiotic factors in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to C. elegans meiotic DSB repair dynamics in the germline. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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