Dynamic Live Cell Imaging of Budding Yeast Meiosis.
| Autor: | Chuong HH; Oklahoma Medical Research Foundation, Oklahoma City, OK, USA., Evatt JM; Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.; Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma City, OK, USA., Dawson DS; Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. dawsond@omrf.org.; Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma City, OK, USA. dawsond@omrf.org. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2818, pp. 161-169. |
| DOI: | 10.1007/978-1-0716-3906-1_10 |
| Abstrakt: | For over a century, major advances in understanding meiosis have come from the use of microscopy-based methods. Studies using the budding yeast, Saccharomyces cerevisiae, have made important contributions to our understanding of meiosis because of the facility with which budding yeast can be manipulated as a genetic model organism. In contrast, imaging-based approaches with budding yeast have been constrained by the small size of its chromosomes. The advent of advances in fluorescent chromosome tagging techniques has made it possible to use yeast more effectively for imaging-based approaches as well. This protocol describes live cell imaging methods that can be used to monitor chromosome movements throughout meiosis in living yeast cells. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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