In Vitro Superparamagnetic Hyperthermia Employing Magnetite Gamma-Cyclodextrin Nanobioconjugates for Human Squamous Skin Carcinoma Therapy.

Autor: Caizer-Gaitan IS; Department of Plastic and Reconstructive Surgery, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania.; Department of Clinical Practical Skills, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania.; Advanced Cardiology and Hemostaseology Research Center, 'Victor Babes' University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania., Watz CG; Department of Pharmaceutical Physics, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania.; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania., Caizer C; Department of Physics, Faculty of Physics, West University of Timisoara, 300223 Timisoara, Romania., Dehelean CA; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania.; Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania., Bratu T; Department of Plastic and Reconstructive Surgery, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania., Crainiceanu Z; Department of Plastic and Reconstructive Surgery, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania., Coroaba A; Center of Advanced Research in Bionanoconjugates and Biopolymers, 'Petru Poni' Institute of Macromolecular Chemistry of Iasi, Romanian Academy, 700487 Iasi, Romania., Pinteala M; Center of Advanced Research in Bionanoconjugates and Biopolymers, 'Petru Poni' Institute of Macromolecular Chemistry of Iasi, Romanian Academy, 700487 Iasi, Romania., Soica CM; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania.; Department of Pharmacology-Pharmacotherapy, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy, 300041 Timisoara, Romania.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Jul 31; Vol. 25 (15). Date of Electronic Publication: 2024 Jul 31.
DOI: 10.3390/ijms25158380
Abstrakt: In vitro alternative therapy of human epidermoid squamous carcinoma (A431) by superparamagnetic hyperthermia (SPMHT) using Fe 3 O 4 (magnetite) superparamagnetic nanoparticles (SPIONs) with an average diameter of 15.8 nm, bioconjugated with hydroxypropyl gamma-cyclodextrins (HP-γ-CDs) by means of polyacrylic acid (PAA) biopolymer, is presented in this paper. The therapy was carried out at a temperature of 43 °C for 30 min using the concentrations of Fe 3 O 4 ferrimagnetic nanoparticles from nanobioconjugates of 1, 5, and 10 mg/mL nanoparticles in cell suspension, which were previously found by us to be non-toxic for healthy cells (cell viabilities close to 100%), according to ISO standards (cell viability must be greater than 70%). The temperature for the in vitro therapy was obtained by the safe application (without exceeding the biological limit and cellular damage) of an alternating magnetic field with a frequency of 312.4 kHz and amplitudes of 168, 208, and 370 G, depending on the concentration of the magnetic nanoparticles. The optimal concentration of magnetic nanoparticles in suspension was found experimentally. The results obtained after the treatment show its high effectiveness in destroying the A431 tumor cells, up to 83%, with the possibility of increasing even more, which demonstrates the viability of the SPMHT method with Fe 3 O 4 -PAA-(HP-γ-CDs) nanobioconjugates for human squamous cancer therapy.
Competing Interests: The authors declare no conflicts of interest.
Databáze: MEDLINE
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