| Autor: |
Alfeqy MM; Pharmacognosy Department, Faculty of Pharmacy, Modern University for Technology & Information, Cairo 11571, Egypt., El-Hawary SS; Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr El Aini, Cairo 11562, Egypt., El-Halawany AM; Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr El Aini, Cairo 11562, Egypt., Rabeh MA; Pharmacognosy Department, College of Pharmacy, King Khalid University, Abha 62251, Saudi Arabia., Alshehri SA; Pharmacognosy Department, College of Pharmacy, King Khalid University, Abha 62251, Saudi Arabia., Abdelmohsen UR; Deraya Center for Scientific Research, Deraya University, New Minia 61111, Egypt.; Pharmacognosy Department, Faculty of Pharmacy, Minia University, Minia 61519, Egypt., Safwat NA; Microbiology & Immunology Department, Faculty of Pharmacy, Modern University for Technology & Information, Cairo 11571, Egypt., Serry AM; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Modern University for Technology & Information, Cairo 11571, Egypt., Fahmy HA; Pharmacognosy Department, Faculty of Pharmacy, Modern University for Technology & Information, Cairo 11571, Egypt., Ezzat MI; Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr El Aini, Cairo 11562, Egypt. |
| Abstrakt: |
Environmentally friendly biosynthesis of silver nanoparticles (AgNPs) from Aeonium arboreum (L.) Webb & Berthel is reported for the first time. The synthesized AgNPs were characterized using UV-Vis, FTIR, TEM, Zeta potential, and XRD analysis, revealing high stability (-29.1 mV), spherical shape, and an average size of 100 nm. The antimicrobial activity levels of both A. arboreum extract and biosynthesized AgNPs were evaluated against five uropathogens ( Staphylococcus aureus , Enterococcus faecalis , Escherichia coli , Pseudomonas aeruginosa , and Candida albicans ). Both the extract and the AgNPs exhibited significant efficacy, particularly against E. coli , with inhibition zones of 27 mm and 30 mm, respectively. LC-MS analysis tentatively identified 11 secondary metabolites in the extract, including quercetin-3-O-glucoside, quercetin-3-O-rhamnoside, myricetin 3-glucoside, and daphneresinol. In silico docking studies revealed promising binding affinities of these metabolites in relation to key enzymes involved in bacterial folate synthesis (dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS)) and DNA replication (DNA gyrase). These findings demonstrate the potential of A. arboreum -based AgNPs and their associated metabolites as a novel therapeutic approach for combating urinary tract infections. Their antimicrobial, antihemolytic, and antibiofilm properties warrant further investigation. |
