Artificial Trabecular Meshwork Structure Combining Melt Electrowriting and Solution Electrospinning.

Autor: Bikuna-Izagirre M; Tissue Engineering Group, Tecnun School of Engineering, University of Navarra, Manuel Lardizabal 13, 20018 San Sebastian, Spain.; Biomedical Engineering Center, University of Navarra, Campus Universitario, 31080 Pamplona, Spain.; T3mPLATE Harry Perkins Institute of Medical Research, QII Medical Center, 6 Verdun St., Nedlands, WA 6009, Australia.; UWA Center of Medical Research, The University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia., Aldazabal J; Tissue Engineering Group, Tecnun School of Engineering, University of Navarra, Manuel Lardizabal 13, 20018 San Sebastian, Spain.; Biomedical Engineering Center, University of Navarra, Campus Universitario, 31080 Pamplona, Spain.; Navarra Institute of Health Research, IdisNA, Calle Irunlarrea 3, 31088 Pamplona, Spain., Moreno-Montañes J; Ophthalmology Department, University of Navarra Clinic, Avenida PIO XII, 31080 Pamplona, Spain., De-Juan-Pardo E; T3mPLATE Harry Perkins Institute of Medical Research, QII Medical Center, 6 Verdun St., Nedlands, WA 6009, Australia.; UWA Center of Medical Research, The University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia., Carnero E; Navarra Institute of Health Research, IdisNA, Calle Irunlarrea 3, 31088 Pamplona, Spain.; Ophthalmology Department, University of Navarra Clinic, Avenida PIO XII, 31080 Pamplona, Spain., Paredes J; Tissue Engineering Group, Tecnun School of Engineering, University of Navarra, Manuel Lardizabal 13, 20018 San Sebastian, Spain.; Biomedical Engineering Center, University of Navarra, Campus Universitario, 31080 Pamplona, Spain.; Navarra Institute of Health Research, IdisNA, Calle Irunlarrea 3, 31088 Pamplona, Spain.
Jazyk: angličtina
Zdroj: Polymers [Polymers (Basel)] 2024 Jul 30; Vol. 16 (15). Date of Electronic Publication: 2024 Jul 30.
DOI: 10.3390/polym16152162
Abstrakt: The human trabecular meshwork (HTM) is responsible for regulating intraocular pressure (IOP) by means of gradient porosity. Changes in its physical properties, like increases in stiffness or alterations in the extracellular matrix (ECM), are associated with increases in the IOP, which is the primary cause of glaucoma. The complexity of its structure limits the engineered models to one-layered and simple approaches, which do not accurately replicate the biological and physiological cues related to glaucoma. Here, a combination of melt electrowriting (MEW) and solution electrospinning (SE) is explored as a biofabrication technique used to produce a gradient porous scaffold that mimics the multi-layered structure of the native HTM. Polycaprolactone (PCL) constructs with a height of 20-710 µm and fiber diameters of 0.7-37.5 µm were fabricated. After mechanical characterization, primary human trabecular meshwork cells (HTMCs) were seeded over the scaffolds within the subsequent 14-21 days. In order to validate the system's responsiveness, cells were treated with dexamethasone (Dex) and the rho inhibitor Netarsudil (Net). Scanning electron microscopy and immunochemistry staining were performed to evaluate the expected morphological changes caused by the drugs. Cells in the engineered membranes exhibited an HTMC-like morphology and a correct drug response. Although this work demonstrates the utility of combining MEW and SE in reconstructing complex morphological features like the HTM, new geometries and dimensions should be tested, and future works need to be directed towards perfusion studies.
Databáze: MEDLINE
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