Vicia ervilia lectin (VEA) has an antibiofilm effect on both Gram-positive and Gram-negative pathogenic bacteria.

Autor: Belfiori B; Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), 06128, Perugia, Italy., Riccioni C; Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), 06128, Perugia, Italy., Pietrella D; Department of Medicine and Surgery, University of Perugia, Piazzale S. Gambuli 1, 06132, Perugia, Italy., Rubini A; Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), 06128, Perugia, Italy., Caceres ME; Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), 06128, Perugia, Italy., Pupilli F; Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), 06128, Perugia, Italy., Bellucci M; Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), 06128, Perugia, Italy. michele.bellucci@cnr.it., De Marchis F; Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), 06128, Perugia, Italy. francesca.demarchis@cnr.it.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2024 Aug 09; Vol. 206 (9), pp. 371. Date of Electronic Publication: 2024 Aug 09.
DOI: 10.1007/s00203-024-04100-6
Abstrakt: Bacterial growing resistance to antibiotics poses a critical threat to global health. This study investigates, for the first time, the antibiofilm properties of Vicia ervilia agglutinin (VEA) from six different V. ervilia accessions against pathogenic bacteria, and the yeast Candida albicans. In the absence of antimicrobial properties, purified VEA significantly inhibited biofilm formation, both in Gram-positive and Gram-negative bacteria, but not in C. albicans. With an inhibitory concentration ranging from 100 to 500 µg/ml, the VEA antibiofilm activity was more relevant against the Gram-positive bacteria Streptococcus aureus and Staphylococcus epidermidis, whose biofilm was reduced up to 50% by VEA purified from accessions #5 and #36. VEA antibiofilm variability between accessions was observed, likely due to co-purified small molecules rather than differences in VEA protein sequences. In conclusion, VEA seed extracts from the accessions with the highest antibiofilm activity could represent a valid approach for the development of an effective antibiofilm agent.
(© 2024. The Author(s).)
Databáze: MEDLINE
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