Label-free, non-contact determination of resting membrane potential using dielectrophoresis.

Autor: Hughes MP; Department of Biomedical Engineering and Biotechnology, Khalifa University of Science and Technology, Abu Dhabi, UAE. Michael.hughes@ku.ac.ae.; Healthcare Engineering Innovation Center, Khalifa University of Science and Technology, Abu Dhabi, UAE. Michael.hughes@ku.ac.ae., Clarke KSP; Centre for Biomedical Engineering, University of Surrey, Guildford, Surrey, GU2 7XH, UK., Hoque R; Centre for Biomedical Engineering, University of Surrey, Guildford, Surrey, GU2 7XH, UK., Griffiths OV; Centre for Biomedical Engineering, University of Surrey, Guildford, Surrey, GU2 7XH, UK., Kruchek EJ; Centre for Biomedical Engineering, University of Surrey, Guildford, Surrey, GU2 7XH, UK., Johnson MP; Department of Biomedical Engineering and Biotechnology, Khalifa University of Science and Technology, Abu Dhabi, UAE., Tariq MH; Department of Biomedical Engineering and Biotechnology, Khalifa University of Science and Technology, Abu Dhabi, UAE., Kohli N; Department of Biomedical Engineering and Biotechnology, Khalifa University of Science and Technology, Abu Dhabi, UAE.; Healthcare Engineering Innovation Center, Khalifa University of Science and Technology, Abu Dhabi, UAE., Lewis R; Department of Comparative Biomedical Sciences, School of Veterinary Medicine, University of Surrey, Guildford, Surrey, GU2 7XH, UK., Labeed FH; Department of Biology, United Arab Emirates University, Al Ain, UAE. f.labeed@uaeu.ac.ae.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Aug 09; Vol. 14 (1), pp. 18477. Date of Electronic Publication: 2024 Aug 09.
DOI: 10.1038/s41598-024-69000-7
Abstrakt: Measurement of cellular resting membrane potential (RMP) is important in understanding ion channels and their role in regulation of cell function across a wide range of cell types. However, methods available for the measurement of RMP (including patch clamp, microelectrodes, and potential-sensitive fluorophores) are expensive, slow, open to operator bias, and often result in cell destruction. We present non-contact, label-free membrane potential estimation which uses dielectrophoresis to determine the cytoplasm conductivity slope as a function of medium conductivity. By comparing this to patch clamp data available in the literature, we have demonstratet the accuracy of this approach using seven different cell types, including primary suspension cells (red blood cells, platelets), cultured suspension cells (THP-1), primary adherent cells (chondrocytes, human umbilical mesenchymal stem cells), and adherent (HeLa) and suspension (Jurkat) cancer cell lines. Analysis of the effect of ion channel inhibitors suggests the effects of pharmaceutical agents (TEA on HeLa; DMSO and neuraminidase on red blood cells) can also be measured. Comparison with published values of membrane potential suggest that the differences between our estimates and values recorded by patch clamp are accurate to within published margins of error. The method is low-cost, non-destructive, operator-independent and label-free, and has previously been shown to allow cells to be recovered after measurement.
(© 2024. The Author(s).)
Databáze: MEDLINE
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