Design of Cryptococcus neoformans multi-epitope vaccine based on immunoinformatics method.

Autor:	Zhou Z; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China., Zhu F; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China., Ma S; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China., Tan C; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China.; Department of Infection Control Center of Xiangya Hospital, Central South University, Changsha, Hunan 410008, China., Yang H; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China., Zhang P; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China., Xu Y; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China., Qin R; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China., Luo Y; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China., Chen J; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China., Pan P; Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Center of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; Clinical Research Center for Respiratory Diseases in Hunan Province, Changsha, Hunan 410008, China.; Hunan Engineering Research Center for Intelligent Diagnosis and Treatment of Respiratory Disease, Changsha, Hunan 410025, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan 410008, China.
Jazyk:	angličtina
Zdroj:	Medical mycology [Med Mycol] 2024 Aug 02; Vol. 62 (8).
DOI:	10.1093/mmy/myae080
Abstrakt:	Cryptococcus neoformans is a widely distributed opportunistic pathogenic fungus. While C. neoformans commonly infects immunocompromised individuals, it can also affect those who are immunocompetent. Transmission of C. neoformans primarily occurs through the respiratory tract, leading to the development of meningitis. The mortality rate of Cryptococcal meningitis is high, and treatment options are limited. Cryptococcus neoformans infections pose a significant public health threat and currently lack targeted and effective response strategies. This study aimed to screen T lymphocyte (cytotoxic T lymphocyte and helper T lymphocyte) and B lymphocyte epitopes derived from four C. neoformans antigens and develop two multi-epitope vaccines by combining them with various adjuvants. Molecular docking results demonstrated that the vaccines bind stably to Toll-like receptor 4 ( and induce innate immunity. The credibility of the molecular docking results was validated through subsequent molecular dynamics simulations. Furthermore, the results of immune simulation analyses underscored the multi-epitope vaccine's capability to effectively induce robust humoral and cellular immune responses within the host organism. These two vaccines have demonstrated theoretical efficacy against C. neoformans infection as indicated by computer analysis. Nevertheless, additional experimental validation is essential to substantiate the protective efficacy of the vaccines. (© The Author(s) 2024. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.)
Databáze:	MEDLINE
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