Molecular structure and functional responses of IgM, IgT and IgD to Flavobacterium covae and Streptococcus iniae infection in Asian seabass (Lates calcarifer Bloch, 1790).

Autor: Uchuwittayakul A; Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand; Center of Excellence in Aquatic Animal Health Management (CE AAHM), Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand. Electronic address: ffisarb@ku.ac.th., Thangsunan P; Division of Biochemistry and Biochemical Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Center of Excellence for Innovation in Chemistry, Research Laboratory on Advanced Materials for Sensor and Biosensor Innovation, Material Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: Pattanapong.t@cmu.ac.th., Thangsunan P; Division of Biochemistry and Biochemical Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Office of Research Administration, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: tbscience@gmail.com., Rodkhum C; Center of Excellence in Fish Infectious Diseases (CE FID), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address: channarong.r@chula.ac.th., Srisapoome P; Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand; Center of Excellence in Aquatic Animal Health Management (CE AAHM), Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand. Electronic address: ffispssp@ku.ac.th.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Oct; Vol. 153, pp. 109823. Date of Electronic Publication: 2024 Aug 08.
DOI: 10.1016/j.fsi.2024.109823
Abstrakt: The Asian seabass (Lates calcarifer) faces significant disease threats, which are exacerbated by intensive farming practices and environmental changes. Therefore, understanding its immune system is crucial. The current study presents a comprehensive analysis of immune-related genes in Asian seabass peripheral blood leukocytes (PBLs) using Iso-seq technology, identifying 16 key pathways associated with 7857 immune-related genes, comprising 634 unique immune-related genes. The research marks the first comprehensive report on the entire immunoglobulin repertoire in Asian seabass, revealing specific characteristics of immunoglobulin heavy chain constant region transcripts, including IgM (Cμ, ighm), IgT (Cτ, ight), and IgD (Cδ, ighd). The study confirms the presence of membrane-bound form, ighm mb , ight mb , ighd mb of IgM, IgT and IgD and secreted form, ighm sc and ight sc of IgM and IgT, respectively, with similar structural patterns and conserved features in amino acids across immunoglobulin molecules, including cysteine residues crucial for structural integrity observed in other teleost species. In response to bacterial infections by Flavobacterium covae (formerly F. columnare genomovar II) and Streptococcus iniae, both secreted and membrane-bound forms of IgM (ighm mb and ighm sc ) and IgT (ight mb and ight sc ) show significant expression, indicating their roles in systemic and mucosal immunity. The expression of membrane-bound form IgD gene, ighd mb , predominantly exhibits targeted upregulation in PBLs, suggesting a regulatory role in B cell-mediated immunity. The findings underscore the dynamic and tissue-specific expression of immunoglobulin repertoires, ighm mb , ighm sc , ight mb , ight sc and ighd mb in Asian seabass, indicating a sophisticated immune response to bacterial pathogens. These findings have practical implications for fish aquaculture, and disease control strategies, serving as a valuable resource for advancing research in Asian seabass immunology.
Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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