An incoherent feed-forward loop involving bHLH transcription factors, Auxin and CYCLIN-Ds regulates style radial symmetry establishment in Arabidopsis.

Autor: Tasker-Brown W; Department of Crop Genetics, John Innes Centre, Norwich, Norfolk, UK., Koh SWH; Department of Cell and Developmental Biology, John Innes Centre, Norwich, Norfolk, UK., Trozzi N; Department of Cell and Developmental Biology, John Innes Centre, Norwich, Norfolk, UK.; Department of Computational and Systems Biology, John Innes Centre, Norwich, Norfolk, UK., Maio KA; Department of Cell and Developmental Biology, John Innes Centre, Norwich, Norfolk, UK., Jamil I; Department of Cell and Developmental Biology, John Innes Centre, Norwich, Norfolk, UK., Jiang Y; Department of Cell and Developmental Biology, John Innes Centre, Norwich, Norfolk, UK., Majda M; Department of Computational and Systems Biology, John Innes Centre, Norwich, Norfolk, UK., Smith RS; Department of Computational and Systems Biology, John Innes Centre, Norwich, Norfolk, UK., Moubayidin L; Department of Crop Genetics, John Innes Centre, Norwich, Norfolk, UK.; Department of Cell and Developmental Biology, John Innes Centre, Norwich, Norfolk, UK.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Aug 09. Date of Electronic Publication: 2024 Aug 09.
DOI: 10.1111/tpj.16959
Abstrakt: The bilateral-to-radial symmetry transition occurring during the development of the Arabidopsis thaliana female reproductive organ (gynoecium) is a crucial biological process linked to plant fertilization and seed production. Despite its significance, the cellular mechanisms governing the establishment and breaking of radial symmetry at the gynoecium apex (style) remain unknown. To fill this gap, we employed quantitative confocal imaging coupled with MorphoGraphX analysis, in vivo and in vitro transcriptional experiments, and genetic analysis encompassing mutants in two bHLH transcription factors necessary and sufficient to promote transition to radial symmetry, SPATULA (SPT) and INDEHISCENT (IND). Here, we show that defects in style morphogenesis correlate with defects in cell-division orientation and rate. We showed that the SPT-mediated accumulation of auxin in the medial-apical cells undergoing symmetry transition is required to maintain cell-division-oriented perpendicular to the direction of organ growth (anticlinal, transversal cell division). In addition, SPT and IND promote the expression of specific core cell-cycle regulators, CYCLIN-D1;1 (CYC-D1;1) and CYC-D3;3, to support progression through the G1 phase of the cell cycle. This transcriptional regulation is repressed by auxin, thus forming an incoherent feed-forward loop mechanism. We propose that this mechanism fine-tunes cell division rate and orientation with the morphogenic signal provided by auxin, during patterning of radial symmetry at the style.
(© 2024 The Author(s). The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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