Single-plant-omics reveals the cascade of transcriptional changes during the vegetative-to-reproductive transition.
| Autor: | Redmond EJ; Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK., Ronald J; Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK.; School of Molecular Biosciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK., Davis SJ; Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK., Ezer D; Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK. |
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| Jazyk: | angličtina |
| Zdroj: | The Plant cell [Plant Cell] 2024 Oct 03; Vol. 36 (10), pp. 4594-4606. |
| DOI: | 10.1093/plcell/koae226 |
| Abstrakt: | Plants undergo rapid developmental transitions, which occur contemporaneously with gradual changes in physiology. Moreover, individual plants within a population undergo developmental transitions asynchronously. Single-plant-omics has the potential to distinguish between transcriptional events that are associated with these binary and continuous processes. Furthermore, we can use single-plant-omics to order individual plants by their intrinsic biological age, providing a high-resolution transcriptional time series. We performed RNA-seq on leaves from a large population of wild-type Arabidopsis (Arabidopsis thaliana) during the vegetative-to-reproductive transition. Though most transcripts were differentially expressed between bolted and unbolted plants, some regulators were more closely associated with leaf size and biomass. Using a pseudotime inference algorithm, we determined that some senescence-associated processes, such as the reduction in ribosome biogenesis, were evident in the transcriptome before a bolt was visible. Even in this near-isogenic population, some variants are associated with developmental traits. These results support the use of single-plant-omics to uncover rapid transcriptional dynamics by exploiting developmental asynchrony. Competing Interests: Conflict of interest statement. None declared. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.) |
| Databáze: | MEDLINE |
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