Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate.

Autor:	Bernardini C; Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy.; Health Sciences and Technologies-Interdepartmental Center for Industrial Research (CIRI-SDV), Alma Mater Studiorum-University of Bologna, Bologna, Italy., Romagnoli N; Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy., Casalini I; Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy., Turba ME; Genefast srl, Forlì, Italy., Spadari A; Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy., Forni M; Health Sciences and Technologies-Interdepartmental Center for Industrial Research (CIRI-SDV), Alma Mater Studiorum-University of Bologna, Bologna, Italy.; Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy., Gentilini F; Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell 'Emilia, Bologna, Italy.
Jazyk:	angličtina
Zdroj:	International journal of veterinary science and medicine [Int J Vet Sci Med] 2024 Aug 07; Vol. 12 (1), pp. 71-80. Date of Electronic Publication: 2024 Aug 07 (Print Publication: 2024).
DOI:	10.1080/23144599.2024.2380586
Abstrakt:	Platelet lysate, derived from platelets, are valuable biological products rich in bioactive molecules. Their use promotes tissue healing and modulates inflammation. However, maintaining the stability and bioactivity of platelet lysate is challenging since they degrade rapidly at room temperature. This study focused on the possibility to confer enhanced stability to freeze-dried equine platelet lysate as an alternative to platelet-rich plasma (PRP). Platelet lysate (PL) was derived from PRP and freeze-dried either as such or using various adjuvants. Primary cell cultures of porcine Vascular Wall-Mesenchymal Stem Cells were treated with different PL formulations, and cell viability was assessed using an MTT assay. Overall, the addition of PL significantly improved cell viability as compared to controls without growth factor supplementation or with foetal bovine serum. Notably, the freeze-drying process maintained the effectiveness of the PL for at least a week. Furthermore, the study revealed that varying the horse as the source of PL could yield varying effects on cell viability. Detailed freeze-drying protocols were established, including freezing, primary drying and secondary drying phases, and the type of adjuvant. This study demonstrated the potential of freeze-dried equine PL as a viable alternative to PRP and highlighted the importance of precise freeze-drying protocols and adjuvants for standardization. Equine PL showed promise for medical treatment in horses, offering advantages such as extended shelf life, ease of handling, and reduced transportation costs, with the potential for broadened therapeutic usage. Competing Interests: No potential conflict of interest was reported by the author(s). (© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)
Databáze:	MEDLINE
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