Evaluation of Safe Margins Using Immunohistochemistry in Oral Squamous Cell Carcinoma.

Autor: Siddiqa A; Department of Oral and Maxillofacial Surgery, Al-Badar Rural Dental College and Hospital, Naganhalli Road, Kalaburagi, Karnataka India., Jivrajani D; Associate Consultant-Apollo Hospital, Bengaluru, Bengaluru, India., Hallur NH; Department of Oral and Maxillofacial Surgery, Al-Badar Rural Dental College and Hospital, Naganhalli Road, Kalaburagi, Karnataka India., Fatima S; Department of Oral and Maxillofacial Surgery, Al-Badar Rural Dental College and Hospital, Naganhalli Road, Kalaburagi, Karnataka India., Kothari C; Department of Oral and Maxillofacial Surgery, Al-Badar Rural Dental College and Hospital, Naganhalli Road, Kalaburagi, Karnataka India., Kuntaraddi SS; Department of Oral and Maxillofacial Surgery, Al-Badar Rural Dental College and Hospital, Naganhalli Road, Kalaburagi, Karnataka India.
Jazyk: angličtina
Zdroj: Journal of maxillofacial and oral surgery [J Maxillofac Oral Surg] 2024 Aug; Vol. 23 (4), pp. 914-917. Date of Electronic Publication: 2024 Jun 25.
DOI: 10.1007/s12663-024-02236-y
Abstrakt: Background: Hematoxylin & Eosin (H & E) stains have been conventionally used to establish the status of safe margins following resection of primary Oral Squamous Cell Carcinoma. Due to non-specificity of this stain, there is a possibility of false negative results. In this study, we have assessed the role of Immunohistochemistry (IHC) in establishing the status of safe margins.
Aim: To compare Hematoxylin & Eosin (H & E) and Immunohistochemistry (IHC) staining in identification of tumor cells in establishing the status of safe margins.
Methodology: This study included 14 cases diagnosed with OSCC. Following resection, the primary lesion was subjected to Histopathological analysis. 2 sets of HP slides were prepared from serial sectioning of the wax block prepared for each of the four margins. Both sets of slides were stained with H &E stain. One set of these slides was further stained with Pan CK marker (IHC) which is a cytokeratin marker to identify tumour cells.
Results: All the slides with H & E staining reported negative for tumor infiltration and 4 slides (3 patients) out of 56 were reported positive with PanCK marker. There was a statistically significant difference in the number of patients with positive margins using IHC as compared to H & E stain.
Conclusion: Immunohistochemistry using PanCK marker proved to be more efficient in the determination of status of safe margins than routine H & E staining.
Competing Interests: Conflict of interestThe authors declare that the research was conducted in the absence of any commercial or financial relationship that could be constructed as a potential conflict of interest.
(© The Association of Oral and Maxillofacial Surgeons of India 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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