Identification of a stress-responsive subregion of the basolateral amygdala in male rats.

Autor: Aukema RJ; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada.; Mathison Centre for Mental Health, University of Calgary, Calgary, AB, T2N 4N1, Canada., Petrie GN; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada.; Mathison Centre for Mental Health, University of Calgary, Calgary, AB, T2N 4N1, Canada., Matarasso AK; Bioengineering, University of Washington, Seattle, WA, 98195, USA.; Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, 98195, USA.; UW Center for the Neurobiology of Addiction, Pain, and Emotion (NAPE), University of Washington, Seattle, WA, 98195, USA., Baglot SL; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada.; Mathison Centre for Mental Health, University of Calgary, Calgary, AB, T2N 4N1, Canada., Molina LA; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada., Füzesi T; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada., Kadhim S; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada.; Mathison Centre for Mental Health, University of Calgary, Calgary, AB, T2N 4N1, Canada., Nastase AS; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada.; Mathison Centre for Mental Health, University of Calgary, Calgary, AB, T2N 4N1, Canada., Rodriguez Reyes I; Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, 98195, USA.; UW Center for the Neurobiology of Addiction, Pain, and Emotion (NAPE), University of Washington, Seattle, WA, 98195, USA., Bains JS; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada., Morena M; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada.; Mathison Centre for Mental Health, University of Calgary, Calgary, AB, T2N 4N1, Canada.; Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, 00185, Italy.; Neuropsychopharmacology Unit, European Center for Brain Research, Santa Lucia Foundation, Rome, 00143, Italy., Bruchas MR; Bioengineering, University of Washington, Seattle, WA, 98195, USA.; Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, 98195, USA.; UW Center for the Neurobiology of Addiction, Pain, and Emotion (NAPE), University of Washington, Seattle, WA, 98195, USA., Hill MN; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada. mnhill@ucalgary.ca.; Mathison Centre for Mental Health, University of Calgary, Calgary, AB, T2N 4N1, Canada. mnhill@ucalgary.ca.; Department of Cell Biology and Anatomy, University of Calgary, Calgary, AB, T2N 4N1, Canada. mnhill@ucalgary.ca.; Department of Psychiatry, University of Calgary, Calgary, AB, T2N 4N1, Canada. mnhill@ucalgary.ca.
Jazyk: angličtina
Zdroj: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology [Neuropsychopharmacology] 2024 Dec; Vol. 49 (13), pp. 1989-1999. Date of Electronic Publication: 2024 Aug 09.
DOI: 10.1038/s41386-024-01927-x
Abstrakt: The basolateral amygdala (BLA) is reliably activated by psychological stress and hyperactive in conditions of pathological stress or trauma; however, subsets of BLA neurons are also readily activated by rewarding stimuli and can suppress fear and avoidance behaviours. The BLA is highly heterogeneous anatomically, exhibiting continuous molecular and connectivity gradients throughout the entire structure. A critical gap remains in understanding the anatomical specificity of amygdala subregions, circuits, and cell types explicitly activated by acute stress and how they are dynamically activated throughout stimulus exposure. Using a combination of topographical mapping for the activity-responsive protein FOS and fiber photometry to measure calcium transients in real-time, we sought to characterize the spatial and temporal patterns of BLA activation in response to a range of novel stressors (shock, swim, restraint, predator odour) and non-aversive, but novel stimuli (crackers, citral odour). We report four main findings: (1) the BLA exhibits clear spatial activation gradients in response to novel stimuli throughout the medial-lateral and dorsal-ventral axes, with aversive stimuli strongly biasing activation towards medial aspects of the BLA; (2) novel stimuli elicit distinct temporal activation patterns, with stressful stimuli exhibiting particularly enhanced or prolonged temporal activation patterns; (3) changes in BLA activity are associated with changes in behavioural state; and (4) norepinephrine enhances stress-induced activation of BLA neurons via the ß-noradrenergic receptor. Moving forward, it will be imperative to combine our understanding of activation gradients with molecular and circuit-specificity.
(© 2024. The Author(s), under exclusive licence to American College of Neuropsychopharmacology.)
Databáze: MEDLINE
Externí odkaz: