Molecular epidemiology of multidrug-resistant Acinetobacter baumannii isolates from a hospital in Nepal.
Autor: | Sakuma M; Department of Microbiology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan., Tohya M; National Institute of Health Sciences, Kawasaki, Kanagawa, Japan., Hishinuma T; Department of Microbiology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan., Sherchand JB; Tribhuvan University, Kathmandu, Nepal., Kirikae T; Juntendo University, Juntendo Advanced Research Institute of Health Science, Bunkyo, Japan., Tada T; Department of Microbiology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan. Electronic address: t-tada@juntendo.ac.jp. |
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Jazyk: | angličtina |
Zdroj: | Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2024 Sep; Vol. 38, pp. 363-367. Date of Electronic Publication: 2024 Aug 06. |
DOI: | 10.1016/j.jgar.2024.07.017 |
Abstrakt: | Objectives: The emergence of multidrug-resistant (MDR) Acinetobacter baumannii has become a serious worldwide medical problem. This study was designed to clarify the genetic and epidemiological properties of MDR A. baumannii clinical isolates. Methods: A total of 66 MDR A. baumannii isolates were obtained from 66 inpatients between May 2019 and February 2020 in a university hospital in Nepal. Whole genomes of these isolates were sequenced using next-generation sequencing. Phylogenetic trees were constructed from single nucleotide polymorphism concatemers. Multilocus sequence typing (MLST) and clonal complex (CC) analysis were conducted, and drug-resistance genes were identified. Results: Of the 66 isolates, 26 harboured a gene encoding NDM-type metallo-β-lactamase, and 55 harboured a gene encoding the 16S rRNA methyltransferase, ArmA. All isolates had point mutations in the quinolone-resistance-determining regions of gyrA and parC. Phylogenetic analysis showed that 55 isolates harboured armA, 26 harboured bla Conclusions: This study indicates that MDR A. baumannii producing carbapenemase and 16S rRNA methyltransferase, with high resistance to carbapenems and/or aminoglycosides, are spreading in medical settings in Nepal. The genetic backgrounds of MDR A. baumannii isolates have shifted to international clone 2 over several years. (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
Databáze: | MEDLINE |
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