Impact of nanosilver surface electronic distributions on serum protein interactions and hemocompatibility.

Autor:	Rivero PS; Department of Chemistry, Universidad Nacional del Sur. INQUISUR-CONICET, B8000CPB, Bahía Blanca, Argentina., Pistonesi DB; Department of Chemistry, Universidad Nacional del Sur. INQUISUR-CONICET, B8000CPB, Bahía Blanca, Argentina., Belén F; Department of Chemistry, Universidad Nacional del Sur. INQUISUR-CONICET, B8000CPB, Bahía Blanca, Argentina., Centurión ME; Department of Chemistry, Universidad Nacional del Sur. INQUISUR-CONICET, B8000CPB, Bahía Blanca, Argentina., Benedini LA; Department of Chemistry, Universidad Nacional del Sur. INQUISUR-CONICET, B8000CPB, Bahía Blanca, Argentina.; Department of Biology, Biochemistry and Pharmacy, Universidad Nacional del Sur, B8000CPB, Bahía Blanca, Argentina., Rauschemberger MB; Department of Biology, Biochemistry and Pharmacy, Universidad Nacional del Sur, B8000CPB, Bahía Blanca, Argentina.; INBIOSUR-CONICET, B8000CPB, Bahía Blanca, Argentina., Messina PV; Department of Chemistry, Universidad Nacional del Sur. INQUISUR-CONICET, B8000CPB, Bahía Blanca, Argentina.
Jazyk:	angličtina
Zdroj:	Nanotechnology [Nanotechnology] 2024 Aug 29; Vol. 35 (46). Date of Electronic Publication: 2024 Aug 29.
DOI:	10.1088/1361-6528/ad6ce2
Abstrakt:	The translation of silver-based nanotechnology 'from bench to bedside' requires a deep understanding of the molecular aspects of its biological action, which remains controversial at low concentrations and non-spherical morphologies. Here, we present a hemocompatibility approach based on the effect of the distinctive electronic charge distribution in silver nanoparticles (nanosilver) on blood components. According to spectroscopic, volumetric, microscopic, dynamic light scattering measurements, pro-coagulant activity tests, and cellular inspection, we determine that at extremely low nanosilver concentrations (0.125-2.5 μ g ml -1 ), there is a relevant interaction effect on the serum albumin and red blood cells (RBCs). This explanation has its origin in the surface charge distribution of nanosilver particles and their electron-mediated energy transfer mechanism. Prism-shaped nanoparticles, with anisotropic charge distributions, act at the surface level, generating a compaction of the native protein molecule. In contrast, the spherical nanosilver particle, by exhibiting isotropic surface charge, generates a polar environment comparable to the solvent. Both morphologies induce aggregation at NPs/bovine serum albumin ≈ 0.044 molar ratio values without altering the coagulation cascade tests; however, the spherical-shaped nanosilver exerts a negative impact on RBCs. Overall, our results suggest that the electron distributions of nanosilver particles, even at extremely low concentrations, are a critical factor influencing the molecular structure of blood proteins' and RBCs' membranes. Isotropic forms of nanosilver should be considered with caution, as they are not always the least harmful. (© 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)
Databáze:	MEDLINE
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