Dual electrochemical signal "signal-on-off" sensor based on CHA-Td-HCR and CRISPR-Cas12a for MUC1 detection.

Autor: Zhang H; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; College of International Education, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China., Gao H; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China., Liu S; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China., Ren X; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; Graduate School of Shanghai University of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China., Que L; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China., Gu X; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China., Rong S; Public Health School, Mudanjiang Medical University, Mudanjiang, 157011, China., Ma H; Public Health School, Mudanjiang Medical University, Mudanjiang, 157011, China., Ruan J; Faculty of Foreign Languages, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China., Miao M; The College of Medical Technology, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China., Qi X; School of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China., Chang D; Department of Clinical Laboratory, The Affiliated Pudong Hospital, Fudan University, Shanghai, 201399, China. Electronic address: dongchang1969@163.com., Pan H; The Affiliated Zhoupu Hospital, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China. Electronic address: panhongzhi@163.com.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Nov 01; Vol. 279, pp. 126665. Date of Electronic Publication: 2024 Aug 03.
DOI: 10.1016/j.talanta.2024.126665
Abstrakt: Mucin 1 (MUC1) is frequently overexpressed in various cancers and is essential for early cancer detection. Current methods to detect MUC1 are expensive, time-consuming, and require skilled personnel. Therefore, developing a simple, sensitive, highly selective MUC1 detection sensor is necessary. In this study, we proposed a novel "signal-on-off" strategy that, in the presence of MUC1, synergistically integrates catalytic hairpin assembly (CHA) with DNA tetrahedron (Td)-based nonlinear hybridization chain reaction (HCR) to enhance the immobilization of electrochemically active methylene blue (MB) on magnetic nanoparticles (MNP), marking the MB signal "on". Concurrently, the activation of CRISPR-Cas12a by isothermal amplification products triggers the cleavage of single-stranded DNA (ssDNA) at the electrode surface, resulting in a reduction of MgAl-LDH@Fc-AuFe-MIL-101 (containing ferrocene, Fc) on the electrode, presenting the "signal-off" state. Both MB and MgAl-LDH@Fc-AuFe-MIL-101 electrochemical signals were measured and analyzed. Assay parameters were optimized, and sensitivity, stability, and linear range were assessed. Across a concentration spectrum of MUC1 spanning from 10 fg/mL to 100 ng/mL, the MB and MgAl-LDH@Fc-AuFe-MIL-101 signals were calibrated with each other, demonstrating a "signal-on-off" dual electrochemical signaling pattern. This allows for the precise and quantitative detection of MUC1 in clinical samples, offering significant potential for medical diagnosis.
Competing Interests: Declaration of competing interest I am authorized on behalf of all the authors of this article to confirm that no author has any conflict of interest to disclose, all authors have approved the version submitted for publication, the work in this article is original and has not been published previously, and the article is not under consideration by any other journal.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: