Raman microscopy allows to follow internalization, subcellular accumulation and fate of iron oxide nanoparticles in cells.

Autor: Rugiel M; Faculty of Physics and Applied Computer Science, AGH University of Krakow, Mickiewicza 30, 30-059 Krakow, Poland., Janik-Olchawa N; Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland., Kowalczyk J; Faculty of Physics and Applied Computer Science, AGH University of Krakow, Mickiewicza 30, 30-059 Krakow, Poland., Pomorska K; Faculty of Physics and Applied Computer Science, AGH University of Krakow, Mickiewicza 30, 30-059 Krakow, Poland., Sitarz M; Faculty of Materials Science and Ceramics, AGH University of Krakow, Mickiewicza 30, 30-059, Krakow, Poland., Bik E; Academic Centre for Materials and Nanotechnology, AGH University of Krakow, Mickiewicza 30, 30-059 Krakow, Poland., Horak D; Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského 2, 162 00, Prague 6, Czech Republic., Babic M; Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského 2, 162 00, Prague 6, Czech Republic., Setkowicz Z; Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland., Chwiej J; Faculty of Physics and Applied Computer Science, AGH University of Krakow, Mickiewicza 30, 30-059 Krakow, Poland. Electronic address: joanna.chwiej@fis.agh.edu.pl.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Dec 15; Vol. 323, pp. 124888. Date of Electronic Publication: 2024 Jul 26.
DOI: 10.1016/j.saa.2024.124888
Abstrakt: An important issue in the context of both potenial toxicity of iron oxide nanoparticles (IONP) and their medical applications is tracking of the internalization process of these nanomaterials into living cells, as well as their localization and fate within them. The typical methods used for this purpose are transmission electron microscopy, confocal fluorescence microscopy as well as light-scattering techniques including dark-field microscopy and flow cytometry. All the techniques mentioned have their advantages and disadvantages. Among the problems it is necessary to mention complicated sample preparation, difficult interpretation of experimental data requiring qualified and experienced personnel, different behavior of fluorescently labeled IONP comparing to those label-free or finally the lack of possibility of chemical composition characteristics of nanomaterials. The purpose of the present investigation was the assessment of the usefulness of Raman microscopy for the tracking of the internalization of IONP into cells, as well as the optimization of this process. Moreover, the study focused on identification of the potential differences in the cellular fate of superparamagnetic nanoparticles having magnetite and maghemite core. The Raman spectra of U87MG cells which internalized IONP presented additional bands which position depended on the used laser wavelength. They occurred at the wavenumber range 1700-2400 cm -1 for laser 488 nm and below the wavenumber of 800 cm -1 in case of laser 532 nm. The intensity of the mentioned Raman bands was higher for the green laser (532 nm) and their position, was independent and not characteristic on the primary core material of IONP (magnetite, maghemite). The obtained results showed that Raman microscopy is an excellent, non-destructive and objective technique that allows monitoring the process of internalization of IONP into cells and visualizing such nanoparticles and/or their metabolism products within them at low exposure levels. What is more, the process of tracking IONP using the technique may be further improved by using appropriate wavelength and power of the laser source.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE