Lipidome and metabolome profiling of longissimus lumborum beef with different ultimate pH and postmortem aging.

Autor: Giovanini de Oliveira Sartori A; Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, SP 13418-900, Brazil., Silva Antonelo D; Lipid Marker Omics Sciences, Pirassununga, SP 13638-407, Brazil., Ribeiro GH; EMBRAPA Instrumentation, São Carlos, SP 13560-970, Brazil., Colnago LA; EMBRAPA Instrumentation, São Carlos, SP 13560-970, Brazil., de Carvalho Balieiro JC; College of Veterinary Medicine and Animal Science, University of Sao Paulo, Pirassununga, SP, 13635-900, Brazil., Francisquine Delgado E; Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, SP 13418-900, Brazil., Contreras Castillo CJ; Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, SP 13418-900, Brazil. Electronic address: ccastill@usp.br.
Jazyk: angličtina
Zdroj: Meat science [Meat Sci] 2024 Nov; Vol. 217, pp. 109621. Date of Electronic Publication: 2024 Aug 03.
DOI: 10.1016/j.meatsci.2024.109621
Abstrakt: The objective of this exploratory study was to assess the changes on lipidome and metabolome profiling of Longissimus lumborum bull muscle with different ultimate pH (pHu) and aging periods. The bull muscles classified as normal, intermediate, or high pHu were collected from a Brazilian commercial slaughterhouse, cut into steaks, individually vacuum-packaged, and aged for 3 days (3-d) or 21 days (21-d) at 2 °C. Muscle extracts were analyzed for the profiles of both lipids, by mass spectrometry (via direct flow-injection), and metabolites, by nuclear magnetic resonance, with downstream multivariate data analysis. As major results, pairwise comparisons identified C12:0 and C14:0 acylcarnitines as potential biomarkers of the intermediate pHu-muscle, which are related to lipid catabolism for alternative energy metabolism and indicate less protein breakage postmortem. Interestingly, the concentration of arginine at early postmortem aging (3-d) may influence the previously reported improved tenderness in normal and high pHu-muscles. Moreover, upregulation of fumarate, formate, and acetate with increased pHu muscle at 21-d aging indicate more intense tricarboxylic acid cycle, amino acid degradation, and pyruvate oxidation by reactive oxygen species, respectively. These three compounds (fumarate, formate, and acetate) discriminated statistically the muscle with high pHu at 21-d aging. The normal pHu-muscle showed higher concentrations of glycogenolysis and glycolysis metabolites, including glucose, mannose, and pyruvate. Hence, our results enhance knowledge of postmortem biochemical changes of beef within different pHu groups aged up to 21 days, which is essential to understand the mechanisms underpinning bull meat quality changes.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Carmen Josefina Contreras Castillo reports financial support was provided by State of Sao Paulo Research Foundation. Alan Giovanini de Oliveira Sartori reports financial support was provided by State of Sao Paulo Research Foundation. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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