Effects of topical isoxsuprine ointment on imiquimod-induced psoriasiform skin inflammation in mice.

Autor: Khafaji AW; Department of Pharmacology, College of Medicine, Al-Nahrain University, Baghdad, Iraq., Al-Zubaidy AA; Department of Pharmacology, College of Medicine, University of Warith Al-Anbiyaa, Karbala, Iraq., Farhood IG; Department of Medicine, College of Medicine, Al-Nahrain University, Baghdad, Iraq., Fawzi HA; Department of Pharmacy, Al-Mustafa University College, Baghdad, Iraq. haider-pharm@almustafauniversity.edu.iq.
Jazyk: angličtina
Zdroj: Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 2024 Aug 08. Date of Electronic Publication: 2024 Aug 08.
DOI: 10.1007/s00210-024-03359-2
Abstrakt: This study assesses the potential positive impact of a 0.05% isoxsuprine ointment on psoriasiform skin inflammation generated by imiquimod in mouse models. Thirty-two male albino mice were allocated into four groups: the control group (which received topical emollients twice daily for 16 days), the induction group (which received imiquimod cream (5%) for 8 days, twice daily followed by petrolatum gel (15%) for another 8 days), and the other two groups, which received imiquimod cream (5%) for 8 days followed by either clobetasol ointment (0.05%) or isoxsuprine ointment (0.05%) twice daily for an additional 8 days. At the end of the experiment, mice were sacrificed by ethical standards, and levels of TNF-α, IL-6, IL-17A, IL-23, and VEGF were measured; PASI and Backer's score were examined, in addition to the histopathology of skin tissue. Each clobetasol and isoxsuprine group displayed a significant reduction in tissue homogenate levels of TNF-α, IL-6, IL-17A, IL-23, and VEGF, besides increments in IL-10 compared to the induction group. Some markers (IL-17A, IL23, and VEGF) showed no significant difference between clobetasol and the isoxsuprine group. In contrast, the other markers (TNF-α, IL6, and IL10) showed significant differences between clobetasol and isoxsuprine groups. Isoxsuprine ointment showed comparable efficacy to clobetasol ointment in treating imiquimod-induced psoriasiform skin inflammation in mice models, probably due to its possible effect of anti-inflammatory and immunomodulatory activities.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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