Visualization and characterization of complement activation in acetylcholine receptor antibody seropositive myasthenia gravis.
Autor: | Huang YF; Department of Medical Sciences, Clinical Neurophysiology, Uppsala University, Uppsala, Sweden., Sandholm K; Linnaeus Center of Biomaterials Chemistry, Linnaeus University, Kalmar, Sweden., Persson B; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Nilsson B; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Punga AR; Department of Medical Sciences, Clinical Neurophysiology, Uppsala University, Uppsala, Sweden. |
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Jazyk: | angličtina |
Zdroj: | Muscle & nerve [Muscle Nerve] 2024 Oct; Vol. 70 (4), pp. 851-861. Date of Electronic Publication: 2024 Aug 08. |
DOI: | 10.1002/mus.28227 |
Abstrakt: | Introduction/aims: There are no blood biomarkers to monitor treatment effects in myasthenia gravis (MG) or studies visualizing the acetylcholine receptor (AChR) antibody-induced membrane attack complex (MAC) at the human muscle membrane. This study aimed to compare levels of complement activation products and native complement components in MG patients and healthy controls (HCs) and to model the AChR antibody-mediated attacks in human muscle cells. Methods: We assessed the complement components and activation product levels with enzyme-linked immunosorbent assay and magnetic bead-based sandwich assays in plasma and sera of 23 MG patients and matched HCs. Receiver operator characteristic (ROC) curve analysis evaluated the diagnostic accuracy. Complement levels were correlated with the myasthenia gravis composite (MGC) scores. AChR+ MG modeling in human muscle cells used sera from nine MG patients and three HCs. Results: MG patients had significantly higher plasma levels of C3a (p < .0001), C5 (p = .0003), and soluble C5b-9 (sC5b-9; p < .0001) than HCs. The ROC curve analysis showed a clear separation between MG patients and HCs for plasma C3a (AUC = 0.9720; p < .0001) and sC5b-9 (AUC = 0.8917, p < .0001). MG patients had higher levels of plasma complement Factor I (FI; p = .0002) and lower properdin levels (p < .0001). The MGC had moderate correlations with plasma Factor B (FB), FI, and Factor H. AChR+ MG patient sera triggered the deposition of MAC and reduced AChRs. Discussion: We suggest validating plasma C3a and sC5b-9 as blood biomarkers for complement activation in MG. Further, the in vitro study allowed visualization of MAC deposition after applying AChR+ MG sera on human muscle cells. (© 2024 The Author(s). Muscle & Nerve published by Wiley Periodicals LLC.) |
Databáze: | MEDLINE |
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