Improving quality control procedures for the measurement of total daily energy expenditure using the two-point doubly labeled water method.

Autor: Breit MJ; Department of Medicine, Division of Endocrinology, Metabolism, and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA., Duncan NM; Department of Medicine, Division of Endocrinology, Metabolism, and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA., Dahle JH; Department of Biology, United States Air Force Academy, Colorado Springs, Colorado, USA., Catenacci VA; Department of Medicine, Division of Endocrinology, Metabolism, and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.; Anschutz Health and Wellness Center, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA., Creasy SA; Department of Medicine, Division of Endocrinology, Metabolism, and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.; Anschutz Health and Wellness Center, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA., Berman ES; Berman Scientific Consulting, Mountain View, California, USA., Speakman JR; Shenzhen Key Laboratory of Metabolic Health, Center for Energy Metabolism and Reproduction, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.; School of Biological Sciences, University of Aberdeen, Aberdeen, Scotland, UK.; China Medical University, Shenyang, Liaoning, China., Melanson EL; Department of Medicine, Division of Endocrinology, Metabolism, and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.; Division of Geriatric Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.
Jazyk: angličtina
Zdroj: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2024 Oct 15; Vol. 38 (19), pp. e9886.
DOI: 10.1002/rcm.9886
Abstrakt: Rationale: The precision of the doubly labeled water (DLW) method is determined by the precision and accuracy of the isotopic measurements. Quality control (QC) procedures to mitigate sample variability require additional measurements if sample duplicates differ more than a factor of instrument precision. We explored the effect of widening QC ranges on total daily energy expenditure (TDEE) determined using the two-point sampling method.
Methods: We screened DLW data from 121 individuals for instances where samples were analyzed more than twice using our existing QC criteria (±2.0 per mil [δ] for 2 H and ±0.5 δ for 18 O). We then applied wider QC ranges for accepting duplicate measures and recalculated TDEE.
Results: Widening the 2 H QC range to ±10.0 δ in samples collected on the first day (most enriched) and to ±5.0 δ in samples collected on the final day (less enriched) produced almost identical mean TDEE compared to the originally calculated TDEE (2684 ± 508 vs. 2687 ± 512 kcal/day, p = 0.40). There was a strong correlation with the originally calculated TDEE (r 2  = 0.97, p < 0.001).
Conclusions: Expanding the 2 H QC range to ±10.0 δ for samples collected on the first day and ±5.0 δ for samples collected on the final day provides similar mean TDEE results. These findings may help DLW labs optimize QC criteria and reduce analytical costs.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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