Consequences of gene editing of PRLR on thermotolerance, growth, and male reproduction in cattle.

Autor: Cuellar CJ; Department of Animal Sciences University of Florida Gainesville Florida USA., Amaral TF; Department of Animal Sciences University of Florida Gainesville Florida USA.; Present address: Genus PLC/ABS São Paulo Brazil., Rodriguez-Villamil P; Acceligen Eagan Minnesota USA.; Present address: Genus plc De Forest Wisconsin USA., Ongaratto F; Acceligen Eagan Minnesota USA.; Present address: University of Wisconsin Biotechnology Center Madison Wisconsin USA., Martinez DO; Department of Animal Sciences University of Florida Gainesville Florida USA.; Department of Large Animal Clinical Sciences, College of Veterinary Medicine University of Florida Gainesville Florida USA., Labrecque R; The Semex Alliance Guelph Ontario Canada., Losano JDA; Department of Animal Sciences University of Florida Gainesville Florida USA., Estrada-Cortés E; Department of Animal Sciences University of Florida Gainesville Florida USA.; Campo Experimental Centro Altos de Jalisco Instituto Nacional de Investigaciones Forestales, Agrícola y Pecuarias Tepatitlán de Morelos Mexico., Bostrom JR; Acceligen Eagan Minnesota USA., Martins K; Acceligen Eagan Minnesota USA., Rae DO; Department of Large Animal Clinical Sciences, College of Veterinary Medicine University of Florida Gainesville Florida USA., Block J; Department of Animal Science University of Wyoming Laramie Wyoming USA., Hoorn QA; Department of Animal Sciences University of Florida Gainesville Florida USA., Daigneault BW; Department of Animal Sciences University of Florida Gainesville Florida USA., Merriam J; The Semex Alliance Guelph Ontario Canada., Lohuis M; The Semex Alliance Guelph Ontario Canada., Dikmen S; Department of Animal Sciences University of Florida Gainesville Florida USA.; Faculty of Veterinary Medicine, Department of Animal Science Bursa Uludag University Bursa Turkey., Bittar JHJ; Department of Large Animal Clinical Sciences, College of Veterinary Medicine University of Florida Gainesville Florida USA., Maia TS; Department of Animal Sciences University of Florida Gainesville Florida USA., Carlson DF; Recombinetics, Inc Eagan Minnesota USA., Larson S; Acceligen Eagan Minnesota USA., Sonstegard TS; Acceligen Eagan Minnesota USA., Hansen PJ; Department of Animal Sciences University of Florida Gainesville Florida USA.
Jazyk: angličtina
Zdroj: FASEB bioAdvances [FASEB Bioadv] 2024 Jun 18; Vol. 6 (8), pp. 223-234. Date of Electronic Publication: 2024 Jun 18 (Print Publication: 2024).
DOI: 10.1096/fba.2024-00029
Abstrakt: Global warming is a major challenge to the sustainable and humane production of food because of the increased risk of livestock to heat stress. Here, the example of the prolactin receptor ( PRLR ) gene is used to demonstrate how gene editing can increase the resistance of cattle to heat stress by the introduction of mutations conferring thermotolerance. Several cattle populations in South and Central America possess natural mutations in PRLR that result in affected animals having short hair and being thermotolerant. CRISPR/Cas9 technology was used to introduce variants of PRLR in two thermosensitive breeds of cattle - Angus and Jersey. Gene-edited animals exhibited superior ability to regulate vaginal temperature (heifers) and rectal temperature (bulls) compared to animals that were not gene-edited. Moreover, gene-edited animals exhibited superior growth characteristics and had larger scrotal circumference. There was no evidence for deleterious effects of the mutation on carcass characteristics or male reproductive function. These results indicate the potential for reducing heat stress in relevant environments to enhance cattle productivity.
Competing Interests: Authors from Acceligen and Semex have an interest in commercialization of gene‐edited livestock. There are no other conflicts.
(©2024 The Authors FASEB BioAdvances published by The Federation of American Societies for Experimental Biology.)
Databáze: MEDLINE
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