The regulatory effects of water probiotic supplementation on the blood physiology, reproductive performance, and its related genes in Red Tilapia (Oreochromis niloticus X O. mossambicus).

Autor: Eissa EH; Fish Research Centre, Faculty of Agricultural Environmental Sciences, Arish University, El-Arish, 45511, Egypt., El-Sayed AM; Oceanography Department, Faculty of Science, Alexandria University, Alexandria, Egypt., Hendam BM; Department of Husbandry and Development of Animal Wealth, Mansoura University, Mansoura, Egypt., Ghanem SF; National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt., Abd Elnabi HE; Department of Fish Resources and Aquaculture, Faculty of Environmental Agricultural Sciences, Arish University, El-Arish, Egypt., Abd El-Aziz YM; Zoology Department, Faculty of Science, Port-Said University, Port Fouad, 42526, Egypt., Abdelnour SA; Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt. saelnour@zu.edu.eg., Eissa MEH; Biotechnology Department, Fish Farming and Technology Institute, Suez Canal University, Ismailia, 41522, Egypt., Dighiesh HS; Department of Aquaculture, Faculty of Fish Resources, Suez University, Suez, 43512, Egypt.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2024 Aug 07; Vol. 20 (1), pp. 351. Date of Electronic Publication: 2024 Aug 07.
DOI: 10.1186/s12917-024-04190-w
Abstrakt: Probiotics are becoming increasingly popular as eco-friendly alternatives in aquaculture. However, there is limited research on their impacts on the reproductive efficiency of Red Tilapia (Oreochromis niloticus x O. mossambicus) broodstock. Therefore, this experiment aimed to explore the combined effects of selective probiotics Bacillus subtilis and B. licheniformis (BSL; 1:1) added to water on blood hematology, serum metabolites, gonadal histology, reproductive performance, and reproductive associated genes in Red Tilapia broodstock. Tilapia broodfish weighing 140-160 g were stocked in four treatment groups: control (T0), and the other three groups were added different levels of BSL to the water as follows: T1 (0.01 g/m 3 ), T2 (0.02 g/m 3 ), and T3 (0.03 g/m 3 ), respectively. Results indicate that BSL administration significantly improved RBCs, hemoglobin, hematocrit, MCH, and MCHC, with the highest improvement seen in the T3 group (P < 0.05). BSL added to the fish water significantly enhanced serum protein fractions (total protein, albumin, and globulins), while AST, ALT, ALP, creatinine, uric acid, and glucose were significantly diminished in a dose-dependent way (P < 0.05). Adding 0.02-0.03 g/ m 3 of BSL resulted in higher antioxidant status (superoxide dismutase and catalase) compared to other groups (P < 0.05). Testosterone levels were higher in T3 than in other groups (P < 0.05). All female hormones (LH, FSH, estradiol, and progesterone) were substantially augmented by the addition of BSL. Additionally, the BSL groups exhibited higher GSI, HSI, VSI (male only), egg diameter (mm), mean number of fry/fish, and mean fry weight (g) compared to the control group (P < 0.05). Expression of reproductive-associated genes (vasa, nanos1a, nanos2, dnd1, pum1, AMH, and vtg) were significantly up-regulated in the gonads of fish in the 0.03 g/m 3 treatment. The histological gonadal structure exhibited that BSL improved gonad maturation in both genders of Tilapia fish. Overall, adding a mixture of B. subtilis and B. licheniformis (0.03 g/m 3 water) can accelerate reproductive performance in Red Tilapia through up-regulation of reproductive genes and enhance the health profile.
(© 2024. The Author(s).)
Databáze: MEDLINE
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