A spatial expression atlas of the adult human proximal small intestine.

Autor: Harnik Y; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Yakubovsky O; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Hoefflin R; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Novoselsky R; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Bahar Halpern K; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Barkai T; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.; Sheba Medical Center, Ramat Gan, Israel., Korem Kohanim Y; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA., Egozi A; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Golani O; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Addadi Y; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Kedmi M; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Keidar Haran T; Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel., Levin Y; The De Botton Institute for Protein Profiling, The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel., Savidor A; The De Botton Institute for Protein Profiling, The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel., Keren-Shaul H; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Mayer C; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.; Institute of Pathology, Sheba Medical Center, Ramat Gan, Israel., Pencovich N; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Pery R; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Shouval DS; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.; Institute of Gastroenterology, Nutrition and Liver Diseases, Schneider Children's Medical Center of Israel, Petach Tikva, Israel., Tirosh I; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Nachmany I; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Itzkovitz S; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel. shalev.itzkovitz@weizmann.ac.il.
Jazyk: angličtina
Zdroj: Nature [Nature] 2024 Aug; Vol. 632 (8027), pp. 1101-1109. Date of Electronic Publication: 2024 Aug 07.
DOI: 10.1038/s41586-024-07793-3
Abstrakt: The mouse small intestine shows profound variability in gene expression along the crypt-villus axis 1,2 . Whether similar spatial heterogeneity exists in the adult human gut remains unclear. Here we use spatial transcriptomics, spatial proteomics and single-molecule fluorescence in situ hybridization to reconstruct a comprehensive spatial expression atlas of the adult human proximal small intestine. We describe zonated expression and cell type representation for epithelial, mesenchymal and immune cell types. We find that migrating enterocytes switch from lipid droplet assembly and iron uptake at the villus bottom to chylomicron biosynthesis and iron release at the tip. Villus tip cells are pro-immunogenic, recruiting γδ T cells and macrophages to the tip, in contrast to their immunosuppressive roles in mouse. We also show that the human small intestine contains abundant serrated and branched villi that are enriched at the tops of circular folds. Our study presents a detailed resource for understanding the biology of the adult human small intestine.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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