Association of Epstein-Barr virus (EBV) with nasopharyngeal carcinoma: Experience from a North Indian tertiary care hospital.

Autor: Biswal D; Department of Microbiology, Amrita Institute of Medical Sciences and Research Centre, Faridabad, Haryana, India. Electronic address: debasishbiswal138@gmail.com., Brijwal M; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India. Electronic address: megha.brijwal@yahoo.com., Choudhary A; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India. Electronic address: aashishpath@yahoo.co.in., Kakkar A; Department of Pathology, All India Institute of Medical Sciences, New Delhi, India. Electronic address: aanchalkakkar@gmail.com., Pramanik R; Department of Medical Oncology, Dr. B.R.A. IRCH, All India Institute of Medical Sciences, New Delhi, India. Electronic address: drrajapramanik@gmail.com., Thakar A; Department of Otolaryngology & Head and Neck Surgery, All India Institute of Medical Sciences, New Delhi, India. Electronic address: drathakar@gmail.com., Dar L; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India. Electronic address: lalitdaraiims@gmail.com.
Jazyk: angličtina
Zdroj: Indian journal of medical microbiology [Indian J Med Microbiol] 2024 Sep-Oct; Vol. 51, pp. 100699. Date of Electronic Publication: 2024 Aug 09.
DOI: 10.1016/j.ijmmb.2024.100699
Abstrakt: Introduction: Nasopharyngeal carcinoma (NPC), arising from nasopharyngeal epithelium is caused by Epstein-Barr virus (EBV). It is common in South China, South East Asia and North East India. The aim and objectives of this study were to determine the prevalence of EBV in formalin-fixed paraffin-embedded (FFPE) tissue sections of clinically suspected NPC patients, correlate the results of polymerase chain reaction (PCR) with histopathology findings, and to determine the utility of tissue EBV DNA as a diagnostic bio-marker.
Materials and Methods: 31 FFPE tissue samples were collected from clinically suspected NPC patients from April 2018-December 2019. Histopathological diagnosis was done by examination of Hematoxylin and Eosin stained slides. Presence of EBV was detected by EBNA-1 PCR. IHC was performed using EBV Latent Membrane Protein 1.
Results: Of the 31 clinically suspected NPC cases, 15 (48.4 %) were histopathological confirmed NPC. Of these15, 13 (86.6 %) were non-keratinising undifferentiated NPC, and one each were keratinising NPC and non-keratinising differentiated NPC respectively. EBV EBNA1 PCR was positive in 35.5 % (11/31) of clinically suspected NPC cases. Of the 11 PCR positive cases, 9 (81.8 %) were histopathological confirmed NPC. Of the 31 clinically suspected NPC cases, IHC was indicated in 23 biopsies. Of which, 12 (52.2 %) were positive for LMP1 in the abnormal cells. Of the 12 IHC positive samples, 10 were NPC cases.
Conclusion: EBV DNA as an indicator towards NPC among clinically suspected cases had a sensitivity of 60 % and specificity of 87.5 %. In this study, addition of EBV DNA detection by PCR from FFPE tissue sections could confirm EBV association in 20 % of cases where it was not detected by EBV LMP1 IHC, thus helped in increasing the detection of EBV positivity in NPC cases. Early diagnosis of NPC will improve the cure rate and hence reduce the morbidity and mortality rates.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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