Separation and purification of bovine nasal cartilage-derived chondroitin sulfate and evaluation of its binding to bovine serum albumin.

Autor: Pang HL; Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China., Zhang LT; Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China., Zhang YT; Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China. Electronic address: zhangyt@mail.jnmc.edu.cn., Ren Q; Department of Pharmacy, Jining Medical University, Rizhao, Shandong, China. Electronic address: renqiang@mail.jnmc.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Oct; Vol. 277 (Pt 4), pp. 134501. Date of Electronic Publication: 2024 Aug 05.
DOI: 10.1016/j.ijbiomac.2024.134501
Abstrakt: This study employs an optimized and environmentally friendly method to extract and purify chondroitin sulfate (CS) from bovine nasal cartilage using enzymatic hydrolysis, ethanol precipitation, and DEAE Sepharose Fast Flow column chromatography. The extracted CS, representing 44.67 % ± 0.0016 of the cartilage, has a molecular weight of 7.62 kDa. Characterization through UV, FT-IR, NMR spectroscopy, and 2-aminoacridone derivatization HPLC revealed a high content of sulfated disaccharides, particularly ΔDi4S (73.59 %) and ΔDi6S (20.61 %). Interaction studies with bovine serum albumin (BSA) using fluorescence spectroscopy and molecular docking confirmed a high-affinity, static quenching interaction with a single binding site, primarily mediated by van der Waals forces and hydrogen bonding. The interaction did not significantly alter the polarity or hydrophobicity of BSA aromatic amino acids. These findings provide a strong foundation for exploring the application of CS in tissue engineering and drug delivery systems, leveraging its unique interaction with BSA for targeted delivery and enhanced efficacy.
Competing Interests: Declaration of competing interest The materials discussed herein have not been published elsewhere before, and the manuscript has not been submitted to any other journals.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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