Microbial communities and functions are structured by vertical geochemical zones in a northern peatland.

Autor: Wang HY; Key Laboratory of Hydrometeorological Disaster Mechanism and Warning, Ministry of Water Resources, School of Hydrology and Water Resources, Nanjing University of Information Science and Technology, Nanjing 210044, China; State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China., Yu ZG; Key Laboratory of Hydrometeorological Disaster Mechanism and Warning, Ministry of Water Resources, School of Hydrology and Water Resources, Nanjing University of Information Science and Technology, Nanjing 210044, China. Electronic address: zhiguo.yu@nuist.edu.cn., Zhou FW; Department of Environmental Science and Engineering, Fudan University, Shanghai, China., Hernandez JC; Department of Microbiology and Biochemistry, University of the Free State, Bloemfontein, South Africa., Grandjean A; University of Münster, Institute for Landscape Ecology, Ecohydrology and Biogeochemistry Group, Heisenbergstr. 2, Münster 48149, Germany., Biester H; Institut für Geoökologie, Technische Universitat Braunschweig, Langer Kamp 19C, Braunschweig 38106, Germany., Xiao KQ; State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China., Knorr KH; University of Münster, Institute for Landscape Ecology, Ecohydrology and Biogeochemistry Group, Heisenbergstr. 2, Münster 48149, Germany. Electronic address: kh.knorr@uni-muenster.de.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Nov 10; Vol. 950, pp. 175273. Date of Electronic Publication: 2024 Aug 05.
DOI: 10.1016/j.scitotenv.2024.175273
Abstrakt: Northern peatlands are important carbon pools; however, differences in the structure and function of microbiomes inhabiting contrasting geochemical zones within these peatlands have rarely been emphasized. Using 16S rRNA gene sequencing, metagenomic profiling, and detailed geochemical analyses, we investigated the taxonomic composition and genetic potential across various geochemical zones of a typical northern peatland profile in the Changbai Mountains region (Northeastern China). Specifically, we focused on elucidating the turnover of organic carbon, sulfur (S), nitrogen (N), and methane (CH 4 ). Three geochemical zones were identified and characterized according to porewater and solid-phase analyses: the redox interface (<10 cm), shallow peat (10-100 cm), and deep peat (>100 cm). The redox interface and upper shallow peat demonstrated a high availability of labile carbon, which decreased toward deeper peat. In deep peat, anaerobic respiration and methanogenesis were likely constrained by thermodynamics, rather than solely driven by available carbon, as the acetate concentrations reached 90 μmol·L -1 . Both the microbial community composition and metabolic potentials were significantly different (p < 0.05) among the redox interface, shallow peat, and deep peat. The redox interface demonstrated a close interaction between N, S, and CH 4 cycling, mainly driven by Thermodesulfovibrionia, Bradyrhizobium, and Syntrophorhabdia metagenome-assembled genomes (MAGs). The archaeal Bathyarchaeia were indicated to play a significant role in the organic carbon, N, and S cycling in shallow peat. Although constrained by anaerobic respiration and methanogenesis, deep peat exhibited a higher metabolic potential for organic carbon degradation, primarily mediated by Acidobacteriota. In terms of CH 4 turnover, subsurface peat (10-20 cm) was a CH 4 production hotspot, with a net turnover rate of ∼2.9 nmol·cm -3 ·d -1 , while the acetoclastic, hydrogenotrophic, and methylotrophic methanogenic pathways all potentially contributed to CH 4 production. The results of this study improve our understanding of biogeochemical cycles and CH 4 turnover along peatland profiles.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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