Detection of the epidemic Pseudomonas aeruginosa AUST-03 (ST242) strain in people with cystic fibrosis in South Africa.

Autor: Hamiwe T; Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa., White DA; Department of Paediatric Pulmonology, Charlotte Maxeke Johannesburg Academic Hospital, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Kwenda S; Division of the National Health Laboratory Service, Sequencing Core Facility, National Institute for Communicable Diseases, Johannesburg, South Africa., Ismail A; Division of the National Health Laboratory Service, Sequencing Core Facility, National Institute for Communicable Diseases, Johannesburg, South Africa.; Department of Biochemistry and Microbiology, University of Venda, Thohoyandou, South Africa.; Institute for Water and Wastewater Technology, Durban University of Technology, Durban, South Africa., Klugman S; Department of Paediatric Pulmonology, Charlotte Maxeke Johannesburg Academic Hospital, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Van Bruwaene L; Department of Paediatric Pulmonology, Steve Biko Academic Hospital/University of Pretoria, Pretoria, South Africa.; Department of Pediatrics, Centre Hospitalier Universitaire Saint-Pierre Hospital, Brussels, Belgium., Goga A; Department of Paediatric Pulmonology, Steve Biko Academic Hospital/University of Pretoria, Pretoria, South Africa.; South African Medical Research Council, Pretoria, South Africa., Kock MM; Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.; Department of Microbiology, National Health Laboratory Service, Tshwane Academic Division, Pretoria, South Africa., Smith AM; Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.; Division of the National Health Laboratory Service, Centre for Enteric Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa., Ehlers MM; Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.; Department of Microbiology, National Health Laboratory Service, Tshwane Academic Division, Pretoria, South Africa.
Jazyk: angličtina
Zdroj: Pediatric pulmonology [Pediatr Pulmonol] 2024 Aug 07. Date of Electronic Publication: 2024 Aug 07.
DOI: 10.1002/ppul.27202
Abstrakt: Introduction: Pseudomonas aeruginosa AUST-03 (ST242) has been reported to cause epidemics in people with CF (pwCF) from Australia and has been associated with multidrug resistance and increased morbidity and mortality. Here, we report an epidemic P. aeruginosa (AUST-03) strain in South African pwCF detected at a public hospital and characterize the genomic antibiotic resistance determinants.
Methods: The P. aeruginosa AUST-03 (ST242) study isolates were analysed with whole genome sequencing using the Illumina NextSeq2000 platform. Raw sequencing reads were processed using the Jekesa pipeline and multilocus sequence typing and genomic antibiotic resistance characterization was performed using public databases. Genetic relatedness between the study isolates and global P. aeruginosa ST242 from public databases was determined using a maximum-likelihood phylogenetic tree. Antibiotic susceptibility testing was performed using the disk diffusion and broth microdilution techniques.
Results: A total of 11 P. aeruginosa AUST-03 isolates were isolated from two children with CF. The majority (8/11) of these isolates were multidrug-resistant (MDR) or extensively drug resistant; and the multidrug efflux pumps MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM were the most clinically relevant antibiotic resistance determinants and were detected in all of the isolates. The study isolates were the most closely related to a 2020 P. aeruginosa AUST-03 (ST242) CF isolate from Russia.
Conclusion: Epidemic MDR P. aeruginosa strains are present at South African public CF clinics and need to be considered when implementing segregation and infection control strategies to prevent possible spread and outbreaks.
(© 2024 The Author(s). Pediatric Pulmonology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: