Transcriptome comparisons of trophoblasts from regenerative cell models with peri-implantation human embryos†.

Autor:	Logsdon DM; Colorado Center for Reproductive Medicine, 10290 RidgeGate Circle, Lone Tree, CO 80124, USA., Ming H; Department of Animal Sciences, Genetics Institute, University of Florida, Gainesville, FL 32610, USA., Ezashi T; Colorado Center for Reproductive Medicine, 10290 RidgeGate Circle, Lone Tree, CO 80124, USA., West RC; Department of Anatomy, Physiology, and Pharmacology, Auburn University, Auburn, AL 36849, USA., Schoolcraft WB; Colorado Center for Reproductive Medicine, 10290 RidgeGate Circle, Lone Tree, CO 80124, USA., Roberts RM; Division of Animal Sciences, University of Missouri-Columbia, MO 65211, USA., Jiang Z; Department of Animal Sciences, Genetics Institute, University of Florida, Gainesville, FL 32610, USA., Yuan Y; Colorado Center for Reproductive Medicine, 10290 RidgeGate Circle, Lone Tree, CO 80124, USA.
Jazyk:	angličtina
Zdroj:	Biology of reproduction [Biol Reprod] 2024 Nov 11; Vol. 111 (5), pp. 1000-1016.
DOI:	10.1093/biolre/ioae120
Abstrakt:	Mechanisms controlling trophoblast (TB) proliferation and differentiation during embryo implantation are poorly understood. Human trophoblast stem cells (TSC) and BMP4/A83-01/PD173074-treated pluripotent stem cell-derived trophoblast cells (BAP) are two widely employed, contemporary models to study TB development and function, but how faithfully they mimic early TB cells has not been fully examined. We evaluated the transcriptomes of TB cells from BAP and TSC and directly compared them with those from peri-implantation human embryos during extended embryo culture (EEC) between embryonic days 8 to 12. The BAP and TSC grouped closely with TB cells from EEC within each TB sublineage following dimensional analysis and unsupervised hierarchical clustering. However, subtle differences in transcriptional programs existed within each TB sublineage. We also validated the presence of six genes in peri-implantation human embryos by immunolocalization. Our analysis reveals that both BAP and TSC models have features of peri-implantation TB s, while maintaining minor transcriptomic differences, and thus serve as valuable tools for studying implantation in lieu of human embryos. (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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