Neuroprotective effects of the combined treatment of resveratrol and urapidil in experimental cerebral ischemia-reperfusion injury in rats.

Autor:	Çetin R; Dicle University - Faculty of Medicine - Department of Neurosurgery - Diyarbakır - Turkey., Bahadir S; Baskent University - Faculty of Medicine - Department of Neurosurgery - Ankara - Turkey., Basar İ; Dicle University - Faculty of Medicine - Department of Neurosurgery - Diyarbakır - Turkey., Aslanoglu B; Dicle University - Faculty of Medicine - Department of Neurosurgery - Diyarbakır - Turkey., Atlas B; Dicle University - Faculty of Medicine - Department of Neurosurgery - Diyarbakır - Turkey., Kaya S; Istanbul Aydin University - Faculty of Medicine - Department of Histology and Embryology - Istanbul - Turkey., Güzel BC; Siirt University - Department of Veterinary Anatomy - Siirt - Turkey., Turan Y; Dicle University - Faculty of Medicine - Department of Neurosurgery - Diyarbakır - Turkey.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2024 Aug 05; Vol. 39, pp. e395329. Date of Electronic Publication: 2024 Aug 05 (Print Publication: 2024).
DOI:	10.1590/acb395329
Abstrakt:	Purpose: To evaluate the neuroprotective effect of resveratrol, urapidil, and a combined administration of these drugs against middle cerebral artery occlusion (MCAO) induced ischemia/reperfusion (IR) injury model in rats. Methods: Thirty-five rats were divided into five groups of seven animals each. Animals in IR, IR resveratrol (IRr), IR urapidil (IRu), and IR + combination of resveratrol and urapidil (IRc) were exposed to MCAO induced cerebral ischemia reperfusion injury model. Rats in IRr and IRu groups received 30-mg/kg resveratrol and 5-mg/kg urapidil respectively. Animals in IRc received a combined treatment of both drugs. At the end of the study, brain tissues were used for oxidative stress (malondialdehyde, glutathione, and superoxide dismutase), pro-apoptotic caspase-3, anti-apoptotic Bcl-2, and pro-inflammatory tumor necrosis factor-α cytokine level measurements. Results: The MCAO model successfully replicated IR injury with significant histopathological changes, elevated tissue oxidative stress, and upregulated apoptotic and inflammatory protein expression in IR group compared to control group (p < 0.001). All parameters were significantly alleviated in IRr group compared to IR group (all p < 0.05). In IRu group, all parameters except for caspase-3 and Bcl-2 were also significantly different than IR group (all p < 0.05). The IRc group showed the biggest difference compared to IR group in all parameters (all p < 0.001). The IRc had higher superoxide dismutase and Bcl-2 levels, and lower caspase-3 levels compared to both IRr and IRu groups (all p < 0.05). Also, the IRc group had lower MDA and TNF-α levels compared to IRu group (all p < 0.05). Conclusions: The results indicate that combined treatment of resveratrol and urapidil may be a novel strategy to downregulate neurodegeneration in cerebral IR injury.
Databáze:	MEDLINE
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