An industrially potent rhamnolipid-like biosurfactant produced from a novel oil-degrading bacterium, Bacillus velezensis S2.

Autor:	Sultana S; Institute of National Analytical Research and Services (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR) New Elephant Road Dhaka 1205 Bangladesh nayeembcsir@gmail.com akborbcsir@gmail.com nasrinakter.nstu@gmail.com shamimbcsir@gmail.com sabinausha@yahoo.com shofiuljamal@yahoo.com.; School of Life and Environmental Sciences, University of Sydney Darlington NSW 2008 Australia ssul0164@uni.sydney.edu.au., Sultana R; Institute of National Analytical Research and Services (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR) New Elephant Road Dhaka 1205 Bangladesh nayeembcsir@gmail.com akborbcsir@gmail.com nasrinakter.nstu@gmail.com shamimbcsir@gmail.com sabinausha@yahoo.com shofiuljamal@yahoo.com.; Medicinal Chemistry and Molecular Pharmacology Department, Purdue University West Lafayette Indiana-47907 USA rsultan@purdue.edu., Al-Mansur MA; Institute of National Analytical Research and Services (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR) New Elephant Road Dhaka 1205 Bangladesh nayeembcsir@gmail.com akborbcsir@gmail.com nasrinakter.nstu@gmail.com shamimbcsir@gmail.com sabinausha@yahoo.com shofiuljamal@yahoo.com., Akbor MA; Institute of National Analytical Research and Services (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR) New Elephant Road Dhaka 1205 Bangladesh nayeembcsir@gmail.com akborbcsir@gmail.com nasrinakter.nstu@gmail.com shamimbcsir@gmail.com sabinausha@yahoo.com shofiuljamal@yahoo.com., Bhuiyan NA; Institute of National Analytical Research and Services (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR) New Elephant Road Dhaka 1205 Bangladesh nayeembcsir@gmail.com akborbcsir@gmail.com nasrinakter.nstu@gmail.com shamimbcsir@gmail.com sabinausha@yahoo.com shofiuljamal@yahoo.com., Ahmed S; Institute of National Analytical Research and Services (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR) New Elephant Road Dhaka 1205 Bangladesh nayeembcsir@gmail.com akborbcsir@gmail.com nasrinakter.nstu@gmail.com shamimbcsir@gmail.com sabinausha@yahoo.com shofiuljamal@yahoo.com., Yasmin S; Institute of National Analytical Research and Services (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR) New Elephant Road Dhaka 1205 Bangladesh nayeembcsir@gmail.com akborbcsir@gmail.com nasrinakter.nstu@gmail.com shamimbcsir@gmail.com sabinausha@yahoo.com shofiuljamal@yahoo.com., Shofiul Islam Molla Jamal AHM; Institute of National Analytical Research and Services (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR) New Elephant Road Dhaka 1205 Bangladesh nayeembcsir@gmail.com akborbcsir@gmail.com nasrinakter.nstu@gmail.com shamimbcsir@gmail.com sabinausha@yahoo.com shofiuljamal@yahoo.com.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2024 Aug 05; Vol. 14 (34), pp. 24516-24533. Date of Electronic Publication: 2024 Aug 05 (Print Publication: 2024).
DOI:	10.1039/d4ra02572e
Abstrakt:	Surfactants can reduce the interfacial surface tension between two immiscible liquids making them a desirable component for various industrial applications. However, the toxic nature of chemical surfactants brought immense attention towards biosurfactants. Being biodegradable, biosurfactants are eco-friendly and considered safer for different commercial uses. This study focused on the production of biosurfactant from an oil-degrading bacteria and its functional efficacy for prospective industrial applications. Here, a promising oil-tolerant strain, Bacillus velezensis S2 was isolated from oil contaminated sites which showed >50% degradation of convoluted crude oil within 28 days in comparison to a control. The isolate was then found to produce an excellent surface-active compound with an emulsification index of 67.30 ± 0.8% and could reduce the surface tension up to 36.86 ± 0.36 mN m -1 . It also manifested a critical micelle concentration of 45 mg L -1 while reducing the surface tension from 72 to 30 mN m -1 . When extracting biosurfactant from isolated bacteria, ethyl acetate extraction showed 1.5 times greater efficacy than chloroform : methanol extraction. The purified biosurfactant was characterized using TLC, 1 H NMR, 13C NMR, FTIR, elemental analyses and spectrophotometric techniques leading to its identification as a rhamnolipid. The stability of produced biosurfactant at higher temperature (up to 180 °C) was determined by thermal analysis, endorsing its application in high temperature reservoir conditions. Additionally, the extracted biosurfactant showed excellent foaming efficacy with insignificant antibacterial and cytotoxic responses, which indicates their potential application in cleaning and cosmetics industries. Thus, the present study outlines a bi-functional novel isolate Bacillus velezensis S2 which could play a significant role in oil remediation from the environment as well as serve as a potential source of non-toxic and eco-friendly biosurfactants for various industrial applications. Competing Interests: The authors declare no conflict of interest. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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