IFN- γ -Licensed Mesenchymal Stem Cells Are More Susceptible to Death when Exposed to Quorum-Sensing Signal Molecule OdDHL and Less Effective in Inhibiting the Growth of Pseudomonas aeruginosa .

Autor: Sousa MRR; Laboratory of Hematology and Stem Cells (LHCT) Health Sciences Department University of Brasilia, Brasília, DF, Brazil.; Molecular Pharmacology Laboratory Health Sciences Department University of Brasília, Brasilia, DF, Brazil., Silva-Carvalho AÉ; Laboratory of Hematology and Stem Cells (LHCT) Health Sciences Department University of Brasilia, Brasília, DF, Brazil., Sousa MGDC; Genomic Sciences and Biotechnology Program Catholic University of Brasília, Brasilia, DF, Brazil.; Division of Biomaterials and Biomechanics Department of Restorative Dentistry School of Dentistry Oregon Health and Science University, Portland, OR, USA.; Knigth Cancer Precision Biofabrication Hub Knigth Cancer Institute Oregon Health and Science University, Portland, OR, USA.; Cancer Early Detection Advanced Research Center Oregon Health Science University, Portland, OR, USA., Martins DCM; Health Sciences Postgraduate Program University of Brasília, Brasília, DF, Brazil.; Dentistry Course Catholic University of Brasília, Brasília, DF, Brazil., Garcez EM; Multidisciplinary Laboratory of Biosciences Faculty of Medicine University of Brasilia, Brasilia, DF, Brazil., Filiú Braga LDC; Laboratory of Hematology and Stem Cells (LHCT) Health Sciences Department University of Brasilia, Brasília, DF, Brazil., de Carvalho JL; Multidisciplinary Laboratory of Biosciences Faculty of Medicine University of Brasilia, Brasilia, DF, Brazil., Dalmolin TV; Clinical Analysis Laboratory Faculty of Health Sciences University of Brasília, Brasília, DF, Brazil., Rezende TMB; Genomic Sciences and Biotechnology Program Catholic University of Brasília, Brasilia, DF, Brazil.; Health Sciences Postgraduate Program University of Brasília, Brasília, DF, Brazil.; Department of Dentistry Faculty of Health Sciences University of Brasília, Brasília, DF, Brazil., Saldanha-Araujo F; Laboratory of Hematology and Stem Cells (LHCT) Health Sciences Department University of Brasilia, Brasília, DF, Brazil.
Jazyk: angličtina
Zdroj: Stem cells international [Stem Cells Int] 2024 Jul 30; Vol. 2024, pp. 2934308. Date of Electronic Publication: 2024 Jul 30 (Print Publication: 2024).
DOI: 10.1155/2024/2934308
Abstrakt: Currently, a series of licensing strategies has been investigated to enhance the functional properties of mesenchymal stem cells (MSCs). Licensing with IFN- γ is one of the most investigated strategies for enhancing the immunosuppressive potential of such cells. However, it is not yet known whether this licensing strategy could interfere with the ability of MSCs to control bacterial growth, which may be relevant considering their clinical potential. In this study, we compared the antimicrobial potential of IFN- γ -licensed and unlicensed MSCs by exposing them to Pseudomonas aeruginosa and its quorum-sensing inducer molecule OdDHL. Our data show that-when challenged with OdDHL-IFN- γ -licensed and unlicensed MSCs present increased levels of the antimicrobial HAMP transcript, but that only IFN- γ -licensed MSCs undergo modulation of CASP1 and BCL2 , entering apoptosis. Furthermore, we demonstrate that only IFN- γ -licensed MSCs show modulation in genes involved in apoptosis and tend to undergo cell death when cultured with P. aeruginosa . As a consequence, IFN- γ -licensed MSCs showed lower capacity to control bacterial growth, compared to unlicensed MSCs. Taken together, our observations reveal an increased susceptibility to apoptosis of IFN- γ -licensed MSCs, which compromises their potential to control the bacterial growth in vitro . These findings are relevant to the field of cell therapy, considering the potential applicability of MSCs.
Competing Interests: The authors declare that they have no competing interests.
(Copyright © 2024 Marielly Reis Resende Sousa et al.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje