Harnessing the power of Neobacillus niacini AUMC-B524 for silver oxide nanoparticle synthesis: optimization, characterization, and bioactivity exploration.

Autor: El-Sapagh SH; Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, 31527, Egypt., El-Zawawy NA; Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, 31527, Egypt., Elshobary ME; Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, 31527, Egypt. mostafa_elshobary@science.tanta.edu.eg., Alquraishi M; Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, 11421, Riyadh, Saudi Arabia., Zabed HM; School of Life Sciences, Guangzhou University, Guangzhou, 510006, Guangdong, China., Nouh HS; Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Jazyk: angličtina
Zdroj: Microbial cell factories [Microb Cell Fact] 2024 Aug 06; Vol. 23 (1), pp. 220. Date of Electronic Publication: 2024 Aug 06.
DOI: 10.1186/s12934-024-02484-0
Abstrakt: Background: Biotechnology provides a cost-effective way to produce nanomaterials such as silver oxide nanoparticles (Ag 2 ONPs), which have emerged as versatile entities with diverse applications. This study investigated the ability of endophytic bacteria to biosynthesize Ag 2 ONPs.
Results: A novel endophytic bacterial strain, Neobacillus niacini AUMC-B524, was isolated from Lycium shawii Roem. & Schult leaves and used to synthesize Ag 2 ONPS extracellularly. Plackett-Burman design and response surface approach was carried out to optimize the biosynthesis of Ag 2 ONPs (Bio-Ag 2 ONPs). Comprehensive characterization techniques, including UV-vis spectral analysis, Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray diffraction, dynamic light scattering analysis, Raman microscopy, and energy dispersive X-ray analysis, confirmed the precise composition of the Ag 2 ONPS. Bio-Ag 2 ONPs were effective against multidrug-resistant wound pathogens, with minimum inhibitory concentrations (1-25 µg mL -1 ). Notably, Bio-Ag 2 ONPs demonstrated no cytotoxic effects on human skin fibroblasts (HSF) in vitro, while effectively suppressing the proliferation of human epidermoid skin carcinoma (A-431) cells, inducing apoptosis and modulating the key apoptotic genes including Bcl-2 associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), Caspase-3 (Cas-3), and guardian of the genome (P53).
Conclusions: These findings highlight the therapeutic potential of Bio-Ag 2 ONPs synthesized by endophytic N. niacini AUMC-B524, underscoring their antibacterial efficacy, anticancer activity, and biocompatibility, paving the way for novel therapeutic strategies.
(© 2024. The Author(s).)
Databáze: MEDLINE
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