Genome and Compound Analysis of Sioxanthin-Producing Marine Actinobacterium Micromonospora sp. nov. Strain SH-82 Isolated from Sponge Scopalina hapalia.

Autor: Ramesh C; Biological Oceanography Division (BOD), National Institute of Oceanography (CSIR-NIO), Dona Paula, Panaji, Goa, 403004, India. chramesh@nio.org.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, 201002, India. chramesh@nio.org., Anwesh M; DBT-Centre for Microbial Informatics, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, Telangana, 500046, India. anwesh023@gmail.com., Alessia T; Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci snc, 98168, Messina, Italy. alessia.tropea@unime.it., Giuffrida D; Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Via Consolare Valeria, 98125, Messina, Italy., La Tella R; Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci snc, 98168, Messina, Italy., Chiaia V; Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci snc, 98168, Messina, Italy., Mondello L; Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci snc, 98168, Messina, Italy.; Chromaleont S.R.L., Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Viale G. Palatucci snc, 98168, Messina, Italy., Anil K; Biological Oceanography Division (BOD), National Institute of Oceanography (CSIR-NIO), Dona Paula, Panaji, Goa, 403004, India., Le Loarer A; Chemistry and Biotechnology of Natural Products, CHEMBIOPRO, Université de La Réunion, Faculté des Sciences et Technologies, 15 Avenue René Cassin, 97744, Saint-Denis CEDEX 9, France., Gauvin-Bialecki A; Chemistry and Biotechnology of Natural Products, CHEMBIOPRO, Université de La Réunion, Faculté des Sciences et Technologies, 15 Avenue René Cassin, 97744, Saint-Denis CEDEX 9, France., Fouillaud M; Chemistry and Biotechnology of Natural Products, CHEMBIOPRO, Université de La Réunion, Faculté des Sciences et Technologies, 15 Avenue René Cassin, 97744, Saint-Denis CEDEX 9, France., Dufossé L; Chemistry and Biotechnology of Natural Products, CHEMBIOPRO, Université de La Réunion, Faculté des Sciences et Technologies, 15 Avenue René Cassin, 97744, Saint-Denis CEDEX 9, France. laurent.dufosse@univ-reunion.fr.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2024 Aug 06; Vol. 81 (9), pp. 298. Date of Electronic Publication: 2024 Aug 06.
DOI: 10.1007/s00284-024-03812-8
Abstrakt: Pigments and other secondary metabolites originating from marine microbes have been a promising natural colorants and drugs for multifaceted applications. However, marine actinobacteria producing such natural molecules are least investigated in terms of their taxonomy, chemical diversity and applications in biomedical, textile, and food industries. In this study, sioxanthin pigment-producing Gram-positive actinobacteria, Micromonospora sp. strain SH-82 was isolated from a marine sponge, Scopalina hapalia, and its whole genome was analyzed. Strain SH-82is a prolific producer of diverse chemical molecules as it produced more compounds on A1 medium with different culture conditions. The genome size of SH-82 is 6.24 Mb (6,246,890 bp) carrying 23 identified biosynthetic gene clusters. A total of 5415 CDS, 60 tRNA, 9 rRNA, and 1 tmRNA are identified from SH-82 genome. The GC content (%) of whole genome was 71.6%. Strain SH-82 harbors genes encoding type I, type II, and type III polyketide synthases. Based on the multi-locus sequence analysis and fatty acid methyl ester (FAME) composition, strain SH-82 is confirmed as a novel species. The genetic information of Micromonospora sp. SH-82 has been deposited to NCBI under the BioProject ID PRJNA1087320, with corresponding identifiers in the Sequence Read Archive (SRA) as SAMN40439676 and the Genome accession as CP148049.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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