| Autor: |
Fulton TL; School of Biological Sciences, Monash University , Clayton, Victoria 3800, Australia., Johnstone JN; School of Biological Sciences, Monash University , Clayton, Victoria 3800, Australia., Tan JJ; School of Biological Sciences, Monash University , Clayton, Victoria 3800, Australia., Balagopal K; School of Biological Sciences, Monash University , Clayton, Victoria 3800, Australia., Dedman A; School of Biological Sciences, Monash University , Clayton, Victoria 3800, Australia., Chan AY; Biomedicine Discovery Institute, Monash University , Clayton, Victoria 3800, Australia., Johnson TK; School of Agriculture, Biomedicine and Environment, La Trobe University , Bundoora, Victoria 3086, Australia., Mirth CK; School of Biological Sciences, Monash University , Clayton, Victoria 3800, Australia., Piper MDW; School of Biological Sciences, Monash University , Clayton, Victoria 3800, Australia. |
| Abstrakt: |
Nutrition and resilience are linked, though it is not yet clear how diet confers stress resistance or the breadth of stressors that it can protect against. We have previously shown that transiently restricting an essential amino acid can protect Drosophila melanogaster against nicotine poisoning. Here, we sought to characterize the nature of this dietary-mediated protection and determine whether it was sex, amino acid and/or nicotine specific. When we compared between sexes, we found that isoleucine deprivation increases female, but not male, nicotine resistance. Surprisingly, we found that this protection afforded to females was not replicated by dietary protein restriction and was instead specific to individual amino acid restriction. To understand whether these beneficial effects of diet were specific to nicotine or were generalizable across stressors, we pre-treated flies with amino acid restriction diets and exposed them to other types of stress. We found that some of the diets that protected against nicotine also protected against oxidative and starvation stress, and improved survival following cold shock. Interestingly, we found that a diet lacking isoleucine was the only diet to protect against all these stressors. These data point to isoleucine as a critical determinant of robustness in the face of environmental challenges. |
