Heme pocket hydrogen bonding residue interactions within the Pectobacterium Diguanylate cyclase-containing globin coupled sensor: A resonance Raman study.

Autor: Hoque NJ; Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA., Rivera S; Department of Chemistry, Emory University, Atlanta, GA 30322, USA., Young PG; Department of Chemistry, Emory University, Atlanta, GA 30322, USA., Weinert EE; Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA. Electronic address: emily.weinert@psu.edu., Liu Y; Department of Chemistry, University of Akron, Akron, OH 44325, USA. Electronic address: yliu1@uakron.edu.
Jazyk: angličtina
Zdroj: Journal of inorganic biochemistry [J Inorg Biochem] 2024 Nov; Vol. 260, pp. 112686. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1016/j.jinorgbio.2024.112686
Abstrakt: Heme-based sensor proteins are used by organisms to control signaling and physiological effects in response to their gaseous environment. Globin-coupled sensors (GCS) are oxygen-sensing proteins that are widely distributed in bacteria. These proteins consist of a heme globin domain linked by a middle domain to various output domains, including diguanylate cyclase domains, which are responsible for synthesizing c-di-GMP, a bacterial second messenger crucial for regulating biofilm formation. To understand the roles of heme pocket residues in controlling activity of the diguanylate cyclase domain, variants of the Pectobacterium carotovorum GCS (PccGCS) were characterized by enzyme kinetics and resonance Raman (rR) spectroscopy. Results of these studies have identified roles for hydrogen bonding and heme edge residues in modulating heme pocket conformation and flexibility. Better understanding of the ligand-dependent GCS signaling mechanism and the residues involved may allow for future development of methods to control O 2 -dependent c-di-GMP production.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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