Amino acid-crosslinked 4arm-PLGA Janus patch with anti-adhesive and anti-bacterial properties for hernia repair.

Autor: Tao Y; State Key Laboratory of Digital Medicine Engineering, Southeast University, Nanjing 210096, PR China; Institute of Medical Devices, Southeast University, Suzhou 215163, PR China., Jiao G; State Key Laboratory of Digital Medicine Engineering, Southeast University, Nanjing 210096, PR China; Institute of Medical Devices, Southeast University, Suzhou 215163, PR China., Zhao X; State Key Laboratory of Digital Medicine Engineering, Southeast University, Nanjing 210096, PR China., Tan X; State Key Laboratory of Digital Medicine Engineering, Southeast University, Nanjing 210096, PR China., Qiao L; State Key Laboratory of Digital Medicine Engineering, Southeast University, Nanjing 210096, PR China., Sheng R; School of Medicine, Southeast University, Nanjing 210009, PR China., Wei D; State Key Laboratory of Digital Medicine Engineering, Southeast University, Nanjing 210096, PR China; Institute of Medical Devices, Southeast University, Suzhou 215163, PR China., Zhang T; State Key Laboratory of Digital Medicine Engineering, Southeast University, Nanjing 210096, PR China; Institute of Medical Devices, Southeast University, Suzhou 215163, PR China. Electronic address: zhangtianzhu@seu.edu.cn.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2024 Nov; Vol. 243, pp. 114126. Date of Electronic Publication: 2024 Aug 02.
DOI: 10.1016/j.colsurfb.2024.114126
Abstrakt: Presently, the non-biodegradable polypropylene (PP) patches frequently used for hernia repair can cause fibrous tissue growth and adhesions. This study created a Janus Patch with anti-adhesion and antimicrobial properties to improve hernia repair while promoting tissue repair. The biologically active 4arm-PLGA-BLPD was initially synthesized through the modification of 4arm-PLGA with lysine, followed by the fabrication of a Janus patch using a layer-by-layer electrostatic spinning technique. This patch consisted of three layers: a repair layer composed of 4arm-PLGA-BLPD/PCL fiber membrane, a mechanical layer of 4arm-PLGA/PCL fiber membrane, and an antimicrobial layer of EMO-4arm-PLGA/PCL fiber membrane loaded with Emodin (EMO). The results showed that Janus patch exhibited notable tensile strength and elongation at break, enabling it to offer enhanced mechanical reinforcement for abdominal wall defects. In addition, it slowly releases lysine for repair and inhibits bacterial growth with EMO. In vivo experiments demonstrated that the patch effectively induced neovascularization, reduced collagen ac-cumulation, and stabilized the expression of relevant proteins through the up-regulation of MMP1 and MMP9. This facilitated successful repair of the abdominal wall defect model and prevented adhesions. In summary, the Janus patch offers both practical application and theoretical insight for hernia repair.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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