Chloroplastic ascorbate modifies plant metabolism and may act as a metabolite signal regardless of oxidative stress.

Autor:	Tóth D; Laboratory for Molecular Photobioenergetics, HUN-REN Biological Research Centre, Institute of Plant Biology, Temesvári krt. 62, Szeged H-6726, Hungary.; Doctoral School of Biology, University of Szeged, Közép fasor 52, Szeged H-6722, Hungary., Tengölics R; HCEMM-BRC Metabolic Systems Biology Lab, Temesvári krt. 62, Szeged H-6726, Hungary.; Synthetic and Systems Biology Unit, HUN-REN Biological Research Centre, Institute of Biochemistry, Temesvári krt. 62, Szeged H-6726, Hungary.; Metabolomics Lab, Core Facilities, HUN-REN Biological Research Centre, Temesvári krt. 62, Szeged H-6726, Hungary., Aarabi F; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm D-14476, Germany., Karlsson A; Science for Life Laboratory, School of Engineering Science in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, PO Box 1031, Solna 171 21, Sweden., Vidal-Meireles A; Laboratory for Molecular Photobioenergetics, HUN-REN Biological Research Centre, Institute of Plant Biology, Temesvári krt. 62, Szeged H-6726, Hungary., Kovács L; Laboratory for Molecular Photobioenergetics, HUN-REN Biological Research Centre, Institute of Plant Biology, Temesvári krt. 62, Szeged H-6726, Hungary., Kuntam S; Laboratory for Molecular Photobioenergetics, HUN-REN Biological Research Centre, Institute of Plant Biology, Temesvári krt. 62, Szeged H-6726, Hungary., Körmöczi T; HCEMM-BRC Metabolic Systems Biology Lab, Temesvári krt. 62, Szeged H-6726, Hungary., Fernie AR; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm D-14476, Germany., Hudson EP; Science for Life Laboratory, School of Engineering Science in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, PO Box 1031, Solna 171 21, Sweden., Papp B; HCEMM-BRC Metabolic Systems Biology Lab, Temesvári krt. 62, Szeged H-6726, Hungary.; Synthetic and Systems Biology Unit, HUN-REN Biological Research Centre, Institute of Biochemistry, Temesvári krt. 62, Szeged H-6726, Hungary.; National Laboratory for Health Security, HUN-REN Biological Research Centre, Temesvári krt. 62, Szeged H-6726, Hungary., Tóth SZ; Laboratory for Molecular Photobioenergetics, HUN-REN Biological Research Centre, Institute of Plant Biology, Temesvári krt. 62, Szeged H-6726, Hungary.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2024 Oct 01; Vol. 196 (2), pp. 1691-1711.
DOI:	10.1093/plphys/kiae409
Abstrakt:	Ascorbate (Asc) is a major plant metabolite that plays crucial roles in various processes, from reactive oxygen scavenging to epigenetic regulation. However, to what extent and how Asc modulates metabolism is largely unknown. We investigated the consequences of chloroplastic and total cellular Asc deficiencies by studying chloroplastic Asc transporter mutant lines lacking PHOSPHATE TRANSPORTER 4; 4 and the Asc-deficient vtc2-4 mutant of Arabidopsis (Arabidopsis thaliana). Under regular growth conditions, both Asc deficiencies caused minor alterations in photosynthesis, with no apparent signs of oxidative damage. In contrast, metabolomics analysis revealed global and largely overlapping alterations in the metabolome profiles of both Asc-deficient mutants, suggesting that chloroplastic Asc modulates plant metabolism. We observed significant alterations in amino acid metabolism, particularly in arginine metabolism, activation of nucleotide salvage pathways, and changes in secondary metabolism. In addition, proteome-wide analysis of thermostability revealed that Asc may interact with enzymes involved in arginine metabolism, the Calvin-Benson cycle, and several photosynthetic electron transport components. Overall, our results suggest that, independent of oxidative stress, chloroplastic Asc modulates the activity of diverse metabolic pathways in vascular plants and may act as an internal metabolite signal. Competing Interests: Conflict of interest statement. None declared. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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