Lab-in-a-Vial Rapid Test for Internet of Things-Embedded Point-of-Healthcare Protein Biomarker Detection in Bodily Fluids.

Autor: Li NS; Department of Biomedical Engineering, National Cheng Kung University, Tainan, 70101, Taiwan., Hsu YP; Department of Materials and Optoelectronic Science, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan., Pang HH; Department of Biomedical Engineering, National Cheng Kung University, Tainan, 70101, Taiwan., Wang SF; Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.; Center for Tropical Medicine and Infectious Diseases Research, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan., Pang ST; Division of Urology, Department of Surgery, Chang Gung Memorial Hospital Linkou, Taoyuan, 33305, Taiwan.; Department of Medicine, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan., Lin CY; Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan., Tsai RY; Caduceus Biotechnology Inc., Hsinchu, 302041, Taiwan., Huang CY; Department of Neurosurgery, Neuroscience Research Center, Chang Gung Memorial Hospital Linkou, Taoyuan, 33305, Taiwan.; Department of Neurosurgery, New Taipei Municipal TuCheng Hospital, Taoyuan, 23652, Taiwan., Wei KC; Department of Medicine, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan.; Department of Neurosurgery, Neuroscience Research Center, Chang Gung Memorial Hospital Linkou, Taoyuan, 33305, Taiwan.; Department of Neurosurgery, New Taipei Municipal TuCheng Hospital, Taoyuan, 23652, Taiwan., Yang HW; Department of Biomedical Engineering, National Cheng Kung University, Tainan, 70101, Taiwan.; Medical Device Innovation Center, National Cheng Kung University, No. 1, University Rd., Tainan City, 70101, Taiwan.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2024 Dec; Vol. 20 (50), pp. e2400878. Date of Electronic Publication: 2024 Aug 06.
DOI: 10.1002/smll.202400878
Abstrakt: Amateurs often struggle with detecting and quantifying protein biomarkers in body fluids due to the high expertise required. This study introduces a Lab-in-a-Vial (LV) rapid diagnostic platform, featuring hydrangea-like platinum nanozymes (PtNH), for rapid, accurate detection and quantification of protein biomarkers on-site within 15 min. This method significantly enhances detection sensitivity for various biomarkers in body fluids, surpassing traditional methods such as enzyme-linked immunosorbent assays (ELISA) and lateral flow assays (LFA) by ≈250 to 1300 times. The LV platform uses a glass vial coated with specific bioreceptors such as antigens or antibodies, enabling rapid in vitro evaluation of disease risk from small fluid samples, similar to a personal ELISA-like point-of-care test (POCT). It overcomes challenges in on-site biomarker detection, allowing both detection and quantification through a portable wireless spectrometer for healthcare internet of things (H-IoT). The platform's effectiveness and adaptability are confirmed using IgG/IgM antibodies from SARS-CoV-2 infected patients and nuclear matrix protein (NMP22) from urothelial carcinoma (UC) patients as biomarkers. These tests demonstrated its accuracy and flexibility. This approach offers vast potential for diverse disease applications, provided that the relevant protein biomarkers in bodily fluids are identified.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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