| Autor: |
Alfaro E; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain., Díaz-García E; Respiratory Service, Centro de Investigación Biomédica en Red de Enfermedades Respiratorias, Madrid, Spain., García-Tovar S; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain., Galera R; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain., Casitas R; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain., Martínez-Cerón E; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain., Torres-Vargas M; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain., Padilla JM; Pneumology Service, Hospital Clínico San Carlos, Madrid, Spain., López-Fernández C; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain., Pérez-Moreno P; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain., Garcia-Rio F; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain., Cubillos-Zapata C; Respiratory Service, Hospital Universitario La Paz, Madrid, Spain. |
| Abstrakt: |
Chronic obstructive pulmonary disease (COPD) is regarded as an accelerated-age disease in which chronic inflammation, maladaptive immune responses and senescence cell burden coexist. Accordingly, cellular senescence has emerged as a potential mechanism involved in COPD pathophysiology. In this study, 25 stable COPD patients underwent a daily physical activity promotion program for six months. We reported that increase of physical activity was related to a reduction of the senescent cell burden in COPD patients' circulating lymphocytes. Senescent T-lymphocytes population, characterized by absence of surface expression of CD28, was reduced after physical activity intervention and the reduction was associated to the increase of physical activity level. In addition, the mRNA expression of cyclin-dependent kinases inhibitors, a hallmark of cell senescence, was reduced and, in accordance, the proliferative capacity of lymphocytes was improved post-intervention. Moreover, we observed an increase in functionality in T-cells from patients after intervention, including improved markers of activation, enhanced cytotoxicity and altered cytokines secretions in response to viral challenge. Lastly, physical activity intervention reduced the potential of lymphocytes' secretome to induce senescence in human primary fibroblasts. In conclusion, our study provides, for the first time, evidence of the potential of physical activity intervention in COPD patients to reduce the senescent burden in circulating immune cells. |
