A genetic method to infer ploidy and aberrant inheritance in triploid organisms.

Autor: Delaval A; Institute of Marine Research, Bergen, Norway., Glover KA; Institute of Marine Research, Bergen, Norway., Solberg MF; Institute of Marine Research, Bergen, Norway., Taggart JB; University of Stirling, Stirling, Scotland, UK., Besnier F; Institute of Marine Research, Bergen, Norway., Sørvik AGE; Institute of Marine Research, Bergen, Norway., Øyro J; Institute of Marine Research, Bergen, Norway., Garnes-Gutvik SN; Institute of Marine Research, Bergen, Norway.; University of Bergen, Bergen, Norway., Fjelldal PG; Institute of Marine Research, Matre Research Station, Matredal, Norway., Hansen T; Institute of Marine Research, Matre Research Station, Matredal, Norway., Harvey A; Institute of Marine Research, Bergen, Norway.
Jazyk: angličtina
Zdroj: Molecular ecology resources [Mol Ecol Resour] 2024 Oct; Vol. 24 (7), pp. e14004. Date of Electronic Publication: 2024 Aug 06.
DOI: 10.1111/1755-0998.14004
Abstrakt: Polyploidy occurs naturally across eukaryotic lineages and has been harnessed in the domestication of many crops and vertebrates. In aquaculture, triploidy can be induced as a biocontainment strategy, as it creates a reproductive barrier preventing farm-to-wild introgression, which is currently a major conservation issue for the industry. However, recent work suggests that triploidisation protocols may, on occasion, produce 'failed triploids' displaying diploidy, aneuploidy and aberrant inheritance. The potentially negative consequences for conservation and animal welfare motivate the need for methods to evaluate the success of ploidy-manipulation protocols early in the production process. We developed a semi-automated version of the MAC-PR (microsatellite DNA allele counting - peak ratios) method to resolve the allelic configuration of large numbers of individuals across a panel of microsatellite markers that can be used to infer ploidy, pedigree and inheritance aberrations. We demonstrate an application of the approach using material from a series of Atlantic salmon (Salmo salar) breeding experiments where ploidy was manipulated using a hydrostatic pressure treatment. We validated the approach to infer ploidy against blood smears, finding a > 99% agreement between these methods, and demonstrate its potential utility to infer ploidy as early as the embryonic stage. Furthermore, we present tools to assign diploid and triploid progeny to families and to detect aberrant inheritance, which may be useful for breeding programmes that utilise ploidy manipulation techniques. The approach adds to the ploidy verification toolbox. The increased precision in detecting ploidy and inheritance aberrations will facilitate the ability of triploidisation programmes to prevent farm-to-wild introgression.
(© 2024 The Author(s). Molecular Ecology Resources published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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