PET/CT Targeted Tissue Sampling Reveals Intravenously Administered HGN194 IgG1 Affects HIV Distribution after Rectal Exposure.

Autor: Taylor RA; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., Xiao S; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., Carias AM; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., McRaven MD; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., Thakkar DN; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., Araínga M; New Iberia Research Center, University of Louisiana at Lafayette, Lafayette, Louisiana, USA., Lorenzo-Redondo R; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.; Center for Pathogen Genomics and Microbial Evolution, Northwestern University Institute for Global Health, Chicago, Illinois, USA., Allen EJ; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., Rogers KA; New Iberia Research Center, University of Louisiana at Lafayette, Lafayette, Louisiana, USA.; Department of Biology, University of Louisiana at Lafayette, Lafayette, Louisiana, USA., Kumarapperuma SC; Research Imaging Institute, University of Texas Health San Antonio, San Antonio, Texas, USA., Gong S; New Iberia Research Center, University of Louisiana at Lafayette, Lafayette, Louisiana, USA.; Department of Microbiology, Immunology, and Molecular Genetics, University of Texas Health San Antonio, San Antonio, Texas, USA.; Texas Biomedical Research Institute and Southwest National Primate Research Center, San Antonio, Texas, USA., Anderson MR; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., Thomas Y; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., Madden PJ; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA., Corti D; Humabs Biomed, a subsidiary of Vir Biotechnology Inc, Bellinzona, Switzerland., Cameroni E; Humabs Biomed, a subsidiary of Vir Biotechnology Inc, Bellinzona, Switzerland., Lanzavecchia A; Humabs Biomed, a subsidiary of Vir Biotechnology Inc, Bellinzona, Switzerland., Goins B; Research Imaging Institute, University of Texas Health San Antonio, San Antonio, Texas, USA., Fox P; Research Imaging Institute, University of Texas Health San Antonio, San Antonio, Texas, USA., Villinger FJ; New Iberia Research Center, University of Louisiana at Lafayette, Lafayette, Louisiana, USA.; Department of Biology, University of Louisiana at Lafayette, Lafayette, Louisiana, USA., Ruprecht RM; New Iberia Research Center, University of Louisiana at Lafayette, Lafayette, Louisiana, USA.; Department of Biology, University of Louisiana at Lafayette, Lafayette, Louisiana, USA.; Department of Microbiology, Immunology, and Molecular Genetics, University of Texas Health San Antonio, San Antonio, Texas, USA.; Texas Biomedical Research Institute and Southwest National Primate Research Center, San Antonio, Texas, USA., Hope TJ; Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
Jazyk: angličtina
Zdroj: AIDS research and human retroviruses [AIDS Res Hum Retroviruses] 2024 Aug 28. Date of Electronic Publication: 2024 Aug 28.
DOI: 10.1089/AID.2024.0019
Abstrakt: Neutralizing monoclonal antibodies hold great potential for prevention of human immunodeficiency virus (HIV) acquisition. IgG is the most abundant antibody in human serum, has a long half-life, and potent effector functions, making it a prime candidate for an HIV prevention therapeutic. We combined Positron Emission Tomography imaging and fluorescent microscopy of 64 Cu-labeled, photoactivatable-green fluorescent protein HIV (PA-GFP-BaL) and fluorescently labeled HGN194 IgG1 to determine whether intravenously instilled IgG influences viral interaction with mucosal barriers and viral penetration in colorectal tissue 2 h after rectal viral challenge. Our results show that IgG1 did not alter the number of virions found throughout the colon or viral penetration into the epithelium of the rectum or descending colon. A minor increase in virions was observed in the transverse colon of IgG1 treated animals. Overall, the number of viral particles found in the mesenteric lymph nodes was low. However, IgG1 administration resulted in a significant reduction of virions found in mesenteric lymph nodes. Taken together, our results show that HGN194 IgG1 does not prevent virions from penetrating into the colorectal mucosa but may perturb HIV virion access to the lymphatic system.
Databáze: MEDLINE