Novel differential scanning calorimetry (DSC) application to select polyhydroxyalkanoate (PHA) producers correlating 3-hydroxyhexanoate (3-HHx) monomer with melting enthalpy.

Autor: Jung HJ; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea., Kim B; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea., Choi TR; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea., Oh SJ; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea., Kim S; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea., Lee Y; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea., Shin Y; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea., Choi S; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea., Oh J; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea., Park SY; Innovation Center, Lotte Chemical LTD, Seoul, Republic of Korea., Lee YS; Innovation Center, Lotte Chemical LTD, Seoul, Republic of Korea., Choi YH; Innovation Center, Lotte Chemical LTD, Seoul, Republic of Korea., Yang YH; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea. seokor@konkuk.ac.kr.; Institute for Ubiquitous Information Technology and Application, Konkuk University, Seoul, Republic of Korea. seokor@konkuk.ac.kr.
Jazyk: angličtina
Zdroj: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2024 Oct; Vol. 47 (10), pp. 1619-1631. Date of Electronic Publication: 2024 Aug 06.
DOI: 10.1007/s00449-024-03054-9
Abstrakt: Polyhydroxyalkanoate (PHA) is an environmental alternative to petroleum-based plastics because of its biodegradability. The polymer properties of PHA have been improved by the incorporation of different monomers. Traditionally, the monomer composition of PHA has been analyzed using gas chromatography (GC) and nuclear magnetic resonance (NMR), providing accurate monomer composition. However, sequential analysis of the thermal properties of PHA using differential scanning calorimetry (DSC) remains necessary, providing crucial insights into its thermal characteristics. To shorten the monomer composition and thermal property analysis, we directly applied DSC to the analysis of the obtained PHA film and observed a high correlation (r 2  = 0.98) between melting enthalpy and the 3-hydroxyhexanoate (3-HHx) mole fraction in the polymer. A higher 3-HHx fraction resulted in a lower melting enthalpy as 3-HHx provided the polymer with higher flexibility. Based on this, we selected the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HHx)) producing strain from Cupriavidus strains that newly screened and transformed with vectors containing P(3HB-co-3HHx) biosynthetic genes, achieving an average error rate below 1.8% between GC and DSC results. Cupriavidus sp. BK2 showed a high 3-HHx mole fraction, up to 10.38 mol%, with T m  (℃) = 171.5 and ΔH of T m (J/g) = 48.0, simultaneously detected via DSC. This study is an example of the expansion of DSC for PHA analysis from polymer science to microbial engineering.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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